Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

Metastatic Lymph Node 51, a novel nucleo-cytoplasmic protein overexpressed in breast cancer

Oncogene volume 21, pages 4422–4434 (2002)Cite this article

Abstract

Metastatic Lymph Node 51 (MLN51) cDNA was isolated by differential screening of a human breast cancer metastasis cDNA library. MLN51 cDNA encodes a novel human protein of 703 residues that shares no significant homology to any known protein. However MLN51 is well conserved between vertebrate and invertebrate species suggesting an important biological function. The amino terminal half of the protein contains a coiled-coil domain and two potential nuclear localization signals (NLS). The carboxy terminal half contains one SH2 and four SH3 binding motifs. The coiled-coil domain promotes MLN51 oligomerization in transfected cells. When transiently expressed, the MLN51 protein is mainly found in the cytoplasm with a weak nuclear staining. However, deletion of the carboxy terminal half of the protein allows the targeting of the protein to the nucleus, demonstrating that the NLSs are functional. MLN51 is ubiquitously expressed in normal tissues. Human breast carcinomas show MLN51 overexpression in malignant epithelial cells. The uncommon association of protein–protein interaction domains often found either in nuclear or in cytoplasmic signaling proteins raises a possible nucleo-cytoplasmic function for MLN51.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 3
Figure 2
Figure 4
Figure 5

Similar content being viewed by others

References

  • Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ . 1997 Nucleic Acids Res. 25: 3389–3402

  • Bairoch A, Bucher P, Hofmann K . 1996 Nucleic Acids Res. 24: 189–196

  • Bieche I, Tomasetto C, Regnier CH, Moog-Lutz C, Rio MC, Lidereau R . 1996 Cancer Res. 56: 3886–3890

  • Boyle P, Maisonneuve P, Autier P . 2000 Int. J. Gynaecol. Obstet. 70: 263–303

  • Burkhard P, Stetefeld J, Strelkov SV . 2001 Trends Cell Biol. 11: 82–88

  • Chen C, Okayama H . 1987 Mol. Cell Biol. 7: 2745–2752

  • Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ . 1979 Biochemistry 18: 5294–5299

  • Chomczynski P, Sacchi N . 1987 Anal. Biochem. 162: 156–159

  • Cohen GB, Ren R, Baltimore D . 1995 Cell 80: 237–248

  • Daigle I, Li C . 1993 Proc. Natl. Acad. Sci. USA 90: 12045–12059

  • Daly RJ . 1998 Cell Signal 10: 613–618

  • Dyrks T, Weidemann A, Multhaup G, Salbaum JM, Lemaire HG, Kang J, Muller-Hill B, Masters CL, Beyreuther K . 1988 EMBO J. 7: 949–957

  • Feinberg AP, Vogelstein B . 1983 Anal. Biochem. 132: 6–13

  • Hansen S, Lane DP, Midgley CA . 1998 J. Mol. Biol. 275: 575–588

  • Hobert O, Jallal B, Schlessinger J, Ullrich A . 1994 J. Biol. Chem. 269: 20225–20228

  • Holt MR, Koffer A . 2001 Trends Cell Biol. 11: 38–46

  • Jans DA, Xiao CY, Lam MH . 2000 Bioessays 22: 532–544

  • Kay BK, Williamson MP, Sudol M . 2000 FASEB J. 14: 231–241

  • Klapper LN, Kirschbaum MH, Sela M, Yarden Y . 2000 Adv. Cancer Res. 77: 25–79

  • Kozak M . 1987 J. Mol. Biol. 196: 947–950

  • Lehrach H, Diamond D, Wozney JM, Boedtker H . 1977 Biochemistry 16: 4743–4751

  • Liotta LA . 2001 Nature 410: 24–25

  • Lupas A . 1996 Methods Enzymol. 266: 513–525

  • Lupas A, Van Dyke M, Stock J . 1991 Science 252: 1162–1164

  • Mayer BJ . 2001 J. Cell Sci. 114: 1253–1263

  • Mayer BJ, Hamaguchi M, Hanafusa H . 1988 Nature 332: 272–275

  • Moog-Lutz C, Tomasetto C, Regnier CH, Wendling C, Lutz Y, Muller D, Chenard MP, Bassett P, Rio MC . 1997 Int. J. Cancer 71: 183–191

  • Nakai K, Horton P . 1999 Trends Biochem. Sci. 24: 34–36

  • O'Shea EK, Rutkowski R, Kim PS . 1992 Cell 68: 699–708

  • Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M . 1997 Cell 90: 373–383

  • Regnier CH, Tomasetto C, Moog-Lutz C, Chenard MP, Wendling C, Bassett P, Rio MC . 1995 J. Biol. Chem. 270: 25715–25721

  • Sadowski I, Stone JC, Pawson T . 1986 Mol. Cell Biol. 6: 4396–4408

  • Schlessinger J . 1994 Curr. Opin. Genet. Dev. 4: 25–30

  • Schreiber V, Moog-Lutz C, Regnier CH, Chenard MP, Boeuf H, Vonesch JL, Tomasetto C, Rio MC . 1998 Mol. Med. 4: 675–687

  • Shoeman RL, Traub P . 1993 Bioessays 15: 605–611

  • Songyang Z, Shoelson SE, Chaudhuri M, Gish G, Pawson T, Haser WG, King F, Roberts T, Ratnofsky S, Lechleider RJ, Neel BG, Birge RB, Farjardo JE, Chou MM, Hanafusa H, Schaffhausen B, Cantley LC . 1993 Cell 72: 767–778

  • Stein D, Wu J, Fuqua SA, Roonprapunt C, Yajnik V, D'Eustachio P, Moskow JJ, Buchberg AM, Osborne CK, Margolis B . 1994 EMBO J. 13: 1331–1340

  • Tanaka S, Mori M, Akiyoshi T, Tanaka Y, Mafune K, Wands JR, Sugimachi K . 1997 Cancer Res. 57: 28–31

  • Taylor SJ, Shalloway D . 1994 Nature 368: 867–871

  • Tomasetto C, Moog-Lutz C, Regnier CH, Schreiber V, Basset P, Rio MC . 1995a FEBS Lett. 373: 245–249

  • Tomasetto C, Regnier C, Moog-Lutz C, Mattei MG, Chenard MP, Lidereau R, Basset P, Rio MC . 1995b Genomics 28: 367–376

  • Wahle E, Keller W . 1992 Ann. Rev. Biochem. 61: 419–440

  • Yan YT, Stein SM, Ding J, Shen MM, Abate-Shen C . 2000 Mol. Cell. Biol. 20: 661–671

  • Yu D, Hung MC . 2000 Oncogene 19: 6115–6121

Download references

Acknowledgements

The authors thank S Chan for critical reading of the manuscript; G Duval, P Eberling, I Stoll, M Gintz for technical assistance; F Alpy, for helpful discussions. This work was supported by funds from the Institut National de la Santé et de la Recherche Médicale, Centre National de la Recherche Scientifique, Hôpital Universitaire de Strasbourg, the Association pour la Recherche sur le Cancer, the Ligue Nationale Française contre le Cancer (LNCC), the LNCC Comités du Haut-Rhin et du Bas-Rhin and the Fondation de France. S Degot was a recipient of an Allocation du Ministère de l'Education Nationale, de la Recherche et de la Technologie.

Author information

Authors and Affiliations

  1. Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), UPR 6520 CNRS/U184 INSERM/Université, Louis Pasteur, BP10142, 67404 Illkirch, C.U., de Strasbourg, France

    Sébastien Degot, Catherine H Régnier, Corinne Wendling, Marie-Christine Rio & Catherine Tomasetto

  2. Service d'Anatomie Pathologique Générale, Centre Hospitalier Universitaire de Hautepierre, Strasbourg, 67098, France

    Marie-Pierre Chenard

Authors
  1. Sébastien Degot
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Catherine H Régnier
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Corinne Wendling
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marie-Pierre Chenard
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Marie-Christine Rio
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Catherine Tomasetto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Catherine Tomasetto.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Degot, S., Régnier, C., Wendling, C. et al. Metastatic Lymph Node 51, a novel nucleo-cytoplasmic protein overexpressed in breast cancer. Oncogene 21, 4422–4434 (2002). https://doi.org/10.1038/sj.onc.1205611

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1205611

Keywords

This article is cited by

Search

Advanced search

Quick links