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  • Oncogenomics
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A comprehensive catalog of CpG islands methylated in human lung adenocarcinomas for the identification of tumor suppressor genes

Oncogene volume 21, pages 3804–3813 (2002)Cite this article

A Corrigendum to this article was published on 02 October 2002

This article has been updated

Abstract

CpG island methylation is an important mechanism in gene silencing and is a key epigenetic event in cancer development. As yet, the number and identities of the genes that are inactivated in cancer cells has not been determined. In order to address this issue, we have performed a comprehensive isolation of CpG islands that are methylated in human lung adenocarcinomas. We have isolated approximately 200 CpG islands that are methylated in tumor DNA including those of known tumor-associated genes such as the HOXA5 gene. As the library contains the CpG islands of a number of known tumor suppressor genes it is highly likely that additional, previously unidentified tumor suppressor genes, will be present. On average, 1–2% of CpG islands were methylated specifically in tumors although this figure differed greatly between patients. This study provides an important resource in the search for genes inactivated in tumors and for the investigation of epigenetic dysregulation of gene expression by CpG island methylation.

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Acknowledgements

This work was supported in part by a Grant-in-Aid for Cancer Research from the Ministry of Health and Welfare of Japan, a Grant-in-Aid for Scientific Research on Priority Areas (C) ‘Medical Genome Science’ from the Ministry of Education, Science, Sports and Culture of Japan (to M Shiraishi), a Grant-in-Aid from the Ministry of Health and Welfare of Japan for the 2nd Term Comprehensive 10-Year Strategy for Cancer Control (to M Shiraishi and T Seikiya), a research Grant on Human Genome and Gene Therapy from the Ministry of Health and Welfare, Japan and a Grant from Takeda Science Foundation (to T Sekya). MJ Terry and YH Chuu were Research Fellows supported by the MIT Japan Program. AJ Oates was a Foreign Research Fellow of the Foundation for Promotion of Cancer Research, Japan.

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Authors and Affiliations

  1. DNA Methylation and Genome Function Project, National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, Tokyo, 104-0045, Japan

    Masahiko Shiraishi, Azumi Sekiguchi, Michael J Terry, Adam J Oates, Yuji Miyamoto, Ying H Chuu, Miyo Munakata & Takao Sekiya

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  1. Masahiko Shiraishi
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  2. Azumi Sekiguchi
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Correspondence to Masahiko Shiraishi.

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Shiraishi, M., Sekiguchi, A., Terry, M. et al. A comprehensive catalog of CpG islands methylated in human lung adenocarcinomas for the identification of tumor suppressor genes. Oncogene 21, 3804–3813 (2002). https://doi.org/10.1038/sj.onc.1205454

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