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Participation of small GTPases Rac1 and Cdc42Hs in myoblast transformation

Oncogene volume 21, pages 2901–2907 (2002)Cite this article

Abstract

We have previously shown that expression of active Rac1 and Cdc4Hs inhibits skeletal muscle cell differentiation. We show here, by bromodeoxyuridine incorporation and cyclin D1 expression, that the expression of active Rac1 and Cdc42Hs but not RhoA impairs cell cycle exit of L6 myoblasts cultured in differentiation medium. Furthermore, expression of activated forms of Rac1 and Cdc42Hs elicits the loss of cell contact inhibition and anchorage-dependent growth as measured by focus forming activity and growth in soft agar. RhoA was once again not found to have this effect. We found a constitutive Rac1 and Cdc42Hs activation in three human rhabdomyosarcoma-derived cell lines, one of the most common causes of solid tumours arising from muscle precursors during childhood. Finally, dominant negative forms of Rac1 and Cdc42Hs inhibit cell proliferation of the RD rhabdomyosarcoma cell line. These data suggest an important role for the small GTPases Rac1 and Cdc42Hs in the generation of skeletal muscle tumours.

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References

  • Carnac G, Primig M, Kitzmann M, Chafey P, Tuil D, Lamb N, Fernandez A . 1998 Mol. Biol. Cell. 9: 1891–1902

  • Chardin P, Yeramian P, Madaule P, Tavitian A . 1985 Int. J. Cancer 35: 647–652

  • Clark GJ, Westwick JK, Der CJ . 1997 J. Biol. Chem. 272: 1677–1681

  • Cuenda A, Cohen P . 1999 J. Biol. Chem. 274: 4341–4346

  • Fritz G, Just I, Kaina B . 1999 Int. J. Cancer 81: 682–687

  • Gallo R, Serafini M, Castellani L, Falcone G, Alema S . 1999 Mol. Biol. Cell. 10: 3137–3150

  • Gauthier RC, Vignal E, Meriane M, Roux P, Montcourier P, Fort P . 1998 Mol. Biol. Cell. 9: 1379–1394

  • Gauthier-Rouviere C, Cavadore JC, Blanchard JM, Lamb NJ, Fernandez A . 1991 Cell Regul. 2: 575–588

  • Heller H, Gredinger E, Bengal E . 2001 J. Biol. Chem. 6: 6–

  • Izawa I, Amano M, Chihara K, Yamamoto T, Kaibuchi K . 1998 Oncogene 17: 2863–2871

  • Joneson T, Bar-Sagi D . 1998 J. Biol. Chem. 273: 17991–17994

  • Khosravi-Far R, Solski PA, Clark GJ, Kinch MS, Der CJ . 1995 Mol. Cell. Biol. 15: 6443–6453

  • Kinch MS, Clark GJ, Der CJ, Burridge K . 1995 J. Cell. Biol. 130: 461–471

  • Kozma R, Ahmed S, Best A, Lim L . 1995 Mol. Cell. Biol. 15: 1942–1952

  • Lassar AB, Skapek SX, Novitch B . 1994 Curr. Opin. Cell. Biol. 6: 788–794

  • Mangues R, Pellicer A . 1992 Semin. Cancer Biol. 3: 229–239

  • Meriane M, Roux P, Primig M, Fort P, Gauthier-Rouviere C . 2000 Mol. Biol. Cell. 11: 2513–2528

  • Mira JP, Benard V, Groffen J, Sanders LC, Knaus UG . 2000 Proc. Natl. Acad. Sci. USA 97: 185–189

  • Olson MF, Ashworth A, Hall A . 1995 Science 269: 1270–1272

  • Puri PL, Wu Z, Zhang P, Wood LD, Bhakta KS, Han J, Feramisco JR, Karin M, Wang JY . 2000 Genes Dev. 14: 574–584

  • Qiu RG, Abo A, McCormick F, Symons M . 1997 Mol. Cell. Biol. 17: 3449–3458

  • Qiu RG, Chen J, McCormick F, Symons M . 1995 Proc. Natl. Acad. Sci. USA 92: 11781–11785

  • Rao SS, Kohtz DS . 1995 J. Biol. Chem. 270: 4093–4100

  • Ridley AJ, Paterson HF, Johnston CL, Diekmann D, Hall A . 1992 Cell 70: 401–410

  • Roux P, Gauthier-Rouviere C, Doucet-Brutin S, Fort P . 1997 Curr. Biol. 7: 629–637

  • Sahai E, Olson MF, Marshall CJ . 2001 EMBO J. 20: 755–766

  • Takano H, Komuro I, Oka T, Shiojima I, Hiroi Y, Mizuno T, Yazaki Y . 1998 Mol. Cell. Biol. 18: 1580–1589

  • Van Aelst L, D'Souza-Schorey C . 1997 Genes Dev. 11: 2295–2322

  • Vandromme M, Gauthier RC, Carnac G, Lamb N, Fernandez A . 1992 J. Cell. Biol. 118: 1489–1500

  • Walsh K, Perlman H . 1997 Curr. Opin. Genet. Dev. 7: 597–602

  • Wei L, Zhou W, Croissant JD, Johansen FE, Prywes R, Balasubramanyam A, Schwartz RJ . 1998 J. Biol. Chem. 273: 30287–30294

  • Westwick JK, Lambert QT, Clark GJ, Symons M, Van Aelst L, Pestell RG, Der CJ . 1997 Mol. Cell. Biol. 17: 1324–1335

  • Wu Z, Woodring PJ, Bhakta KS, Tamura K, Wen F, Feramisco JR, Karin M, Wang JY, Puri PL . 2000 Mol. Cell. Biol. 20: 3951–3964

  • Zetser A, Gredinger E, Bengal E . 1999 J. Biol. Chem. 274: 5193–5200

  • Zhong C, Kinch MS, Burridge K . 1997 Mol. Biol. Cell. 8: 2329–2344

  • Zohn IM, Campbell SL, Khosravi-Far R, Rossman KL, Der CJ . 1998 Oncogene 17: 1415–1438

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Acknowledgements

We thank Anne Blangy and Emmanuel Vignal for constant support. We also thank J Collard and M Schwartz for GST-Pak and GST-Rhotekin, respectively and A Eychene for GST-RafGDSRBD. We would like to thank Atta Behfar for critically reading the manuscript. This work was supported by institutional grants from the Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Medicale, and contracts from the Association Francaise pour la Recherche contre le Cancer (ARC contrat n°5568), the Ligue Nationale Contre le Cancer (‘équipe labellisée’) and the Association Francaise contre les myopathies.

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  1. Centre de Recherche de Biochimie Macromoléculaire (CRBM), CNRS UPR 1086, 1919 Route de Mende, 34293 Montpellier Cedex, France

    Mayya Meriane, Sophie Charrasse, Franck Comunale, Annabelle Méry, Philippe Fort, Pierre Roux & Cécile Gauthier-Rouvière

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  1. Mayya Meriane
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Correspondence to Cécile Gauthier-Rouvière.

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Meriane, M., Charrasse, S., Comunale, F. et al. Participation of small GTPases Rac1 and Cdc42Hs in myoblast transformation. Oncogene 21, 2901–2907 (2002). https://doi.org/10.1038/sj.onc.1205396

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