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MGEA6 is tumor-specific overexpressed and frequently recognized by patient-serum antibodies

Oncogene volume 21, pages 239–247 (2002)Cite this article

Abstract

Tumorigenesis of meningioma has been associated with chromosome 22, most notably the NF2 gene, but additional genes have also been implicated in meningioma development. Previously, we have cloned the cDNAs for the meningioma expressed antigen 6 (MGEA6) and its splice variant MGEA11. Here, we show that antibodies against recombinantly expressed MGEA6/11 are found in 41.7% (10/24) of the sera from meningioma patients and in 2/8 sera of glioblastoma patients, whereas no response was seen in 12 sera from healthy persons. Western-blot analyses using generated polyclonal antibodies, revealed overexpression in meningioma and glioma tumor samples compared to normal brain. Immunohistochemical staining of tissue sections confirms reactivity in meningioma tumor cells and tumor cells of glial origin. We found no reactivity to normal astrocytes and only faint reactivity to normal leptomeninges. Sequence analysis predicted membranic localization of MGEA6/11, that was confirmed by cell fractionation. The immune response to MGEA6/11 is frequent in both meningioma and glioma patients and may likely be attributed to overexpression of the MGEA6/11 protein in the tumor cells.

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Acknowledgements

The authors thank Prof Dr WI Steudel and the staff of the Neurosurgical Clinic for providing fresh tumor samples. We thank Mr Marc Voss, Department of Virology, for the excellent help preparing the cell fractions. The skilled technical assistance of Mr W Förderer, Mr P Krück, Mrs S Leyes, Mrs B Simon and Mr B Stock, Department of Neuropathology, is greatly appreciated. This work was supported by a grant from the Deutsche Forschungsgemeinschaft (SFB 399, A4).

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Authors and Affiliations

  1. Institut für Humangenetik, Universitätskliniken des Saarlandes, Homburg/Saar, 66421, Germany

    Nicole Comtesse, Brenda Glass, Dirk Heckel, Esther Maldener & Eckart Meese

  2. Abteilung für Neuropathologie, Universitätskliniken des Saarlandes, Homburg/Saar, 66421, Germany

    Isolde Niedermayer & Wolfgang Feiden

  3. Institut für Medizinische Biochemie, Universitätskliniken des Saarlandes, Homburg/Saar, 66421, Germany

    Wolfgang Nastainczyk

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  1. Nicole Comtesse
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Correspondence to Eckart Meese.

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Comtesse, N., Niedermayer, I., Glass, B. et al. MGEA6 is tumor-specific overexpressed and frequently recognized by patient-serum antibodies. Oncogene 21, 239–247 (2002). https://doi.org/10.1038/sj.onc.1205005

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