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Tumorigenic N-terminal deletions of c-Myb modulate DNA binding, transactivation, and cooperativity with C/EBP

Oncogene volume 20, pages 7420–7424 (2001)Cite this article

Abstract

Oncogenic activation of c-myb by retroviral insertion has been implicated in tumor formation in chicken and mice. These genetic alterations result in deregulated expression of the c-myb gene and frequently in N-terminal truncation of the c-Myb protein. We demonstrate that truncation of the c-Myb N-terminus affects DNA binding and reporter activation. However, all three mutants, MybΔN20, MybΔN47 and MybΔN71 cooperated with C/EBPβ in reporter assays. In contrast to MybΔN20 and MybΔN47, however, the MybΔN71 mutant failed to activate the chromatin embedded endogenous mim-1 gene together with C/EBPβ. This suggests that an N-terminal region (amino acids 47–71) within repeat 1 (R1) of the murine c-Myb DNA binding domain affects activation of chromosomal target genes in collaboration with C/EBPβ.

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References

  • Brendeford EM, Myrset AH, Hegvold AB, Lundin M, Gabrielsen OS . 1997 J. Biol. Chem. 272: 4436–4443

    Article  CAS  Google Scholar 

  • Burk O, Mink S, Ringwald M, Klempnauer K-H . 1993 EMBO J. 12: 2027–2038

  • Dash AB, Orrico FC, Ness SA . 1996 Genes and Dev. 10: 1858–1869

  • Dini PW, Lipsick JS . 1993 Mol. Cell. Biol. 13: 7334–7348

    Article  CAS  Google Scholar 

  • Dini PW, Eltman JT, Lipsick JS . 1995 J. Virol. 69: 2515–2524

  • Ebneth A, Adermann K, Wolfes H . 1994 FEBS Lett. 337: 265–268

  • Ganter B, Lipsick JS . 1999 Adv. Cancer Res. 76: 21–60

  • Introna M, Golay J, Frampton J, Nakano T, Ness SA, Graf T . 1990 Cell 63: 1289–1297

    Article  CAS  Google Scholar 

  • Jiang W, Kanter MR, Dunkel I, Ramsay RG, Beemon KL, Hayward WS . 1997 J. Virol. 71: 6526–6533

  • Kanter MR, Smith RE, Hayward WS . 1988 J. Virol. 62: 1423–1432

  • Katz S, Kowenz-Leutz E, Müller C, Meese K, Ness SA, Leutz A . 1993 EMBO J. 12: 1321–1332

  • Kowenz-Leutz E, Leutz A . 1999 Mol. Cell 4: 735–743

  • Kowenz-Leutz E, Herr P, Niss K, Leutz A . 1997 Cell 91: 185–195

    Article  CAS  Google Scholar 

  • Kowenz-Leutz E, Twamley G, Ansieau S, Leutz A . 1994 Genes and Dev. 8: 2781–2791

  • Kozak M . 1991 J. Cell Biol. 115: 887–903

  • Krieg J, Oelgeschläger M, Janknecht R, Lüscher B . 1995 Oncogene 10: 2221–2228

  • Lüscher B, Christenson E, Litchfield DW, Krebs EG, Eisenman RN . 1990 Nature 344: 517–522

    Article  Google Scholar 

  • Mink S, Haenig B, Klempnauer K-H . 1997 Mol. Cell. Biol. 17: 6609–6617

    Article  CAS  Google Scholar 

  • Mukhopadhyaya R, Wolff L . 1992 J. Virol. 66: 6035–6044

  • Nakano T, Graf T . 1992 Oncogene 7: 527–534

  • Nazarov V, Wolff L . 1995 J. Virol. 69: 3885–3888

  • Ness SA . 1996 Biochim. Biophys. Acta 1288: F123–F139

  • Ness SA, Kowenz-Leutz E, Casini T, Graf T, Leutz A . 1993 Genes and Dev. 7: 749–759

  • Ness SA, Marknell A, Graf T . 1989 Cell 59: 1115–1125

    Article  CAS  Google Scholar 

  • Nomura T, Sakai N, Sarai A, Sudo T, Kanei-Ishii C, Ramsay RG, Favier D, Gonda TJ, Ishii S . 1993 J. Biol. Chem. 268: 21914–21923

  • Oelgeschläger M, Janknecht R, Krieg J, Schreek S, Lüscher B . 1996 EMBO J. 15: 2771–2780

  • Oelgeschläger M, Krieg J, Lüscher-Firzlaff JM, Lüscher B . 1995 Mol. Cell. Biol. 15: 5966–5974

    Article  Google Scholar 

  • Ording E, Kvåvik W, Bostad A, Gabrielsen OS . 1994 Eur. J. Biochem. 222: 113–120

  • Pizer E, Baba TW, Humphries EH . 1992 J. Virol. 66: 512–523

  • Pizer E, Humphries EH . 1989 J. Virol. 63: 1630–1640

  • Saikumar P, Murali R, Reddy EP . 1990 Proc. Natl. Acad. Sci. USA 87: 8452–8459

  • Shen-Ong GLC, Wolff L . 1987 J. Virol. 61: 3721–3725

  • Tanikawa J, Yasukawa T, Enari M, Ogata K, Nishimura Y, Ishii S, Sarai A . 1993 Proc. Natl. Acad. Sci. USA 90: 9320–9324

  • Wolff L . 1996 Crit. Rev. Oncog. 7: 245–260

  • Wolff L, Koller R, Davidson W . 1991 J. Virol. 65: 3607–3616

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Acknowledgements

We thank L Wolff and M Austen for providing reagents. This work was supported by grants from the DFG (Lu 466/1-4) and the Fonds der Chemischen Industrie to B Lüscher.

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Author notes

  1. Michael Oelgeschläger

    Present address: Howard Hughes Medical Institute and Department of Biological Chemistry, University of California, 5748 MacDonald Building, 675 Circle Drive South, Box 951662, Los Angeles, CA, 90095-1662, USA

  2. Michael Oelgeschläger and Elisabeth Kowenz-Leutz: M Oelgeschläger and E Kowenz-Leutz contributed equally to this article

Authors and Affiliations

  1. Institut für Molekularbiologie, Medizinische Hochschule Hannover, Hannover, 30623, Germany

    Michael Oelgeschläger, Sabine Schreek & Bernhard Lüscher

  2. Max-Delbrück-Centrum für Molekulare Medizin, Robert-Roessle-Str. 10, Berlin, 13122, Germany

    Elisabeth Kowenz-Leutz & Achim Leutz

  3. Graffinity GmbH, Im Neuenheimer Feld 515, Heidelberg, 69120, Germany

    Sabine Schreek

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  1. Michael Oelgeschläger
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  2. Elisabeth Kowenz-Leutz
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Correspondence to Bernhard Lüscher.

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Oelgeschläger, M., Kowenz-Leutz, E., Schreek, S. et al. Tumorigenic N-terminal deletions of c-Myb modulate DNA binding, transactivation, and cooperativity with C/EBP. Oncogene 20, 7420–7424 (2001). https://doi.org/10.1038/sj.onc.1204922

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