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Regulation of Laloo by the Xenopus C-terminal Src kinase (Xcsk) during early vertebrate development

Oncogene volume 20, pages 5210–5214 (2001)Cite this article

Abstract

Mesoderm formation in the frog, Xenopus laevis, is dependent on the activity of one or more members of the Src family kinases; the molecular interactions underlying this requirement are not well understood. The C-terminal Src Kinase (Csk) is a potent inhibitor of Src activity, and is required for normal mammalian development; here we report the characterization of Xenopus Csk (Xcsk). Xcsk is widely expressed during early development, physically interacts with the Src kinase Laloo, and inhibits the generation of mesoderm by the Src kinases. Xcsk activity requires a functional kinase domain; furthermore, a kinase-inactive Xcsk mutant potently synergizes with Laloo during early vertebrate development, suggesting a fundamental role for the Src kinase-Csk regulatory circuit during mesoderm induction, in vivo.

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Acknowledgements

The authors thank D Morgan for the gift of Xcsk, R Steele for Xfyn, and J Hama and P Wilson for critical reading of the manuscript. D Weinstein is supported by an Irma T Hirschl Career Scientist Award, by the Speaker's Fund for Biomedical Research: Toward the Science of Patient Care, awarded by the City of New York, and by the AMDeC Foundation of New York City, through its ‘Tartikoff/Perelman/EIF Fund for Young Investigators in Women's Cancers’.

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  1. Department of Pharmacology, Mount Sinai School of Medicine, New York, NY, USA

    Yi Song, Ari N Cohler & Daniel C Weinstein

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  1. Yi Song
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  2. Ari N Cohler
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  3. Daniel C Weinstein
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Correspondence to Daniel C Weinstein.

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Song, Y., Cohler, A. & Weinstein, D. Regulation of Laloo by the Xenopus C-terminal Src kinase (Xcsk) during early vertebrate development. Oncogene 20, 5210–5214 (2001). https://doi.org/10.1038/sj.onc.1204672

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