Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Short Report
  • Published:

Expression, cellular distribution and protein binding of the glioma amplified sequence (GAS41), a highly conserved putative transcription factor

Oncogene volume 20, pages 4853–4863 (2001)Cite this article

Abstract

The glioma amplified sequence 41 (GAS41) was previously isolated by microdissection mediated cDNA capture from the glioblastoma multiforme cell line TX3868 and shown to be frequently amplified in human gliomas. We determined the complete cDNA sequence of the GAS41 gene, demonstrated that the GAS41 protein is evolutionarily conserved, specifically at the N-terminus, and identified the yeast transcription factor tf2f domain within the GAS41 sequence. A human multiple-tissue Northern blot revealed ubiquitous expression of GAS41 with the highest expression in human brain. After generating polyclonal antibodies we found GAS41 protein expression in the nucleus of the TX3868 cell line by Western blot analysis and immunofluorescence microscopy. The nuclear localization was confirmed for several human tumors including gliomas of different grades of malignancy. In neuroblastoma however, GAS41 was found in the nucleoli but not in the nucleoplasm. Yeast two-hybrid screening of the TX3868 cell line identified the nuclear mitotic apparatus protein (NuMA), the KIAA1009 protein, and prefoldin subunit 1 (PFDN1) as potential interacting partners of GAS41. We generated a polyclonal antibody against the KIAA1009 protein and we demonstrated that the KIAA1009 protein is a nuclear protein, which appears to be co-localized with the GAS41 protein and NuMA.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9

Similar content being viewed by others

References

  • Adams MD, Celniker SE, Gibbs RA, Rubin GM, Venter CJ . 2000 Science 287: 2185–2195

  • Albrecht EB, Hunyady AB, Stark GR, Patterson TE . 2000 Mol. Biol. Cell. 11: 873–886

  • Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ . 1997 Nuc. Acids. Res. 25: 3389–3402

  • Barlev NA, Candau R, Wang L, Darpino P, Silverman N, Berger SL . 1995 J. Biol. Chem. 270: 19337–19344

  • Cairns BR, Henry NL, Kornberg RD . 1996 Mol. Cell. Biol. 16: 3308–3316

  • Carlson M, Laurent BC . 1994 Curr. Opin. Cell. Biol. 6: 396–402

  • Compton DA, Luo C . 1995 J. Cell. Sci. 108 Pt 2 621–633

  • Dingwall AK, Beek SJ, McCallum CM, Tamkun JW, Kalpana GV, Goff SP, Scott MP . 1995 Mol. Biol. Cell. 6: 777–791

  • Dionne MA, Howard L, Compton DA . 1999 Cell. Motil. Cytoskeleton 42: 189–203

  • Fischer U, Heckel D, Michel A, Janka M, Hulsebos T, Meese E . 1997 Hum. Mol. Genet. 6: 1817–1822

  • Fischer U, Meltzer P, Meese E . 1996 Hum. Genet. 98: 625–628

  • FlyBase 1999 Nucleic. Acids Res. 27: 85–88.http://flybase.bio.indiana.edu/

  • Gueth-Hallonet C, Wang J, Harborth J, Weber K, Osborn M . 1998 Exp. Cell. Res. 243: 434–452

  • Hansen WJ, Cowan N, Welch WJ . 1999 J. Cell. Biol. 145: 265–277

  • Harlow E, Lane D . 1989 Antibodies. A Laboratory Manual, Cold Spring Harbor Laboratory: USA

    Google Scholar 

  • Harborth J, Weber K, Osborn M . 2000 J. Biol. Chem. 275: 31979–31985

  • Heiskanen MA, Bittner ML, Chen Y, Khan J, Adler KE, Trent JM, Meltzer PS . 2000 Cancer Res. 60: 799–802

  • Jahnknecht R, Hunter T . 1996 Nature 383: 22–23

  • Kleihues P, Soylemezoglu F, Schauble B, Scheithauer BW, Burger PC . 1995 Glia 15: 211–221

  • Livingstone LR, White A, Sprouse J, Livanos E, Jacks T, Tlsty TD . 1992 Cell 70: 923–935

  • Luderus ME, den Blaauwen JL, de Smit OJ, Compton DA, van Driel R . 1994 Mol. Cell. Biol. 14: 6297–6305

  • Lyderson BK, Pettijohn DE . 1980 Cell 22 Pt 2 498–499

  • Martin KJ . 1991 BioEssays 13: 113–123

  • Merrifield RB . 1963 J. Am. Chem. Soc. 85: 2149–2158

  • Mitchell PJ, Tjian R . 1989 Science 245: 371–378

  • Murakami Y, Tateyama S, Rungsipipat A, Uchida K, Yamaguchi R . 2000 J. Vet. Med. Sci. 62: 783–787

  • Näär AM, Beaurang PA, Zhou S, Abraham S, Solomon W, Tjian R . 1999 Nature 398: 828–832

  • Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O . 1999 DNA Res. 6: 63–70

  • Nakamura T, Adler H, Gu Y, Prasad R, Canaani O, Kamada N, Gale RP, Lange B, Crist WM, Nowell PC, Croce CM, Canaani E . 1993 Proc. Natl. Acad. Sci. USA 90: 4631–4635

  • Remacle JE, Albrecht G, Brys R, Braus GH, Huylebroeck D . 1997 EMBO J. 16: 5722–5729

  • Richard AW, Catzavelos C, Kamel-Reid S . 1997 Nature Genetics 17: 109–113

  • Sehgal A . 1998 Semin. Surg. Oncol. 14: 3–12

  • Suzuki SO, Iwaki T . 2000 Mod. Pathol. 13: 548–553

  • Vainberg IE, Lewis SA, Rommelaere H, Ampe C, Vadekerckhove J, Klein HK, Cowan NJ . 1998 Cell 93: 863–873

  • Wang ZH, Ding MX, Chew-Cheng SB, Yun JP, Chew EC . 1999 Anticancer Res. 19: 5445–5449

  • Wang W, Cote J, Xue Y, Zhou S, Khavari PA, Biggar SR, Muchardt C, Kalpana GV, Goff SP, Yaniv M, Workman JL, Crabtree GRv . 1996 EMBO J. 15: 5370–5382

  • Zeng C . 2000 Microscopy Res. Technique. 49: 467–477

Download references

Acknowledgements

The authors thank Dr WI Steudel and the staff of the Neurosurgical Clinic for providing fresh tumor samples, Dr Norbert Schuster, Department of Anatomy, for the kind gift of the nucleolin-specific antibody and the neuroblastoma cell lines and Dr Jens Meyer for critical reading of the manuscript. This work was supported by a Grant from the Deutsche Forschungsgemeinschaft (SFB 399, A1).

Author information

Author notes

  1. A Munnia and N Schütz: A Munnia and N Schütz contributed equally to this work

  2. U Fischer and E Meese: U Fischer and E Meese equal contribution as senior authors

Authors and Affiliations

  1. Institut für Humangenetik, Universitätskliniken des Saarlandes, Homburg/Saar, 66421, Germany

    A Munnia, N Schütz, E Maldener, B Glass, R Maas, U Fischer & E Meese

  2. Abteilung für Neuropathologie, Universitätskliniken des Saarlandes, Homburg/Saar, 66421, Germany

    B FM Romeike & W Feiden

  3. Institut für Medizinische Biochemie, Universitätskliniken des Saarlandes, Homburg/Saar, 66421, Germany

    W Nastainczyk

Authors
  1. A Munnia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N Schütz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. B FM Romeike
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E Maldener
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B Glass
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. R Maas
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. W Nastainczyk
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. W Feiden
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. U Fischer
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. E Meese
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E Meese.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Munnia, A., Schütz, N., Romeike, B. et al. Expression, cellular distribution and protein binding of the glioma amplified sequence (GAS41), a highly conserved putative transcription factor. Oncogene 20, 4853–4863 (2001). https://doi.org/10.1038/sj.onc.1204650

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1204650

Keywords

This article is cited by

Search

Advanced search

Quick links