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Characterization of the promoter region of the viral interferon regulatory factor encoded by Kaposi's sarcoma-associated herpesvirus

Oncogene volume 20, pages 523–530 (2001)Cite this article

Abstract

Viral interferon regulatory factor (vIRF) encoded by Kaposi's sarcoma-associated herpesvirus (KSHV) inhibits the expression of interferon-responsive genes, causes cellular transformation and transactivates KSHV genes. In the present study, we characterized the mRNA expression pattern of the vIRF gene and its promoter. A vIRF transcript of 1.7 kb in size was detected in low level in uninduced KSHV-infected cells and its expression was inducible by 12-O-tetradecanoylphorbol-13-acetate (TPA), sensitive to cycloheximide and resistant to phosphonoacetic acid. The transcription start site was mapped to 79 nt upstream of the ATG initiation site by 5′-RACE. Mutagenesis analysis identified a region between −56 and the transcription start site (+1) as the minimal promoter region that contains a functional TATA box at −27. A region between −337 and −125 contains a repressor domain negated by sequence from −991 to −499 in BCBL-1 cells, a region which was also identified to be responsive to TPA induction. These results demonstrate vIRF as a KSHV early gene, identify its promoter and define the promoter regions that contain regulatory elements controlling vIRF transcription.

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References

  • Asubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA and Struhl K (eds) . 1995 Current Protocols in Molecular Biology. John Wiley and Son: New York.

    Google Scholar 

  • Boshoff C, Whitby D, Hatziionnou T, Fisher C, van der Walt J, Hatzakis A, Weiss R and Schulz T. . 1995 Lancet 345: 1043–1044.

  • Burysek L, Yeow WS, Lubyova B, Kellum M, Schafer SL, Huang YQ and Pitha PM. . 1999 J. Virol. 73: 7334–7342.

  • Carbone A, Gloghini A, Vaccher E, Zagonel V, Pastore C, Dalla Palma P, Branz F, Saglio G, Volpe R, Tirelli U and Gaidano G. . 1996 Br. J. Haematol. 94: 533–543.

  • Cesarman E, Chang Y, Moore PS, Said JW and Knowles DM. . 1995a N. Engl. J. Med. 332: 1186–1191.

  • Cesarman E, Moore PS, Rao PH, Inghirami G, Knowles DM and Chang Y. . 1995b Blood 86: 2708–2714.

  • Chadburn A, Cesarman E, Nador RG, et al. 1996 Lab. Invest. 74: 109A.

  • Chang Y, Cesarman E, Pessin MS, Lee F, Culpepper J, Knowles DM and Moore PS. . 1994 Science 266: 1865–1869.

  • Chang Y, Ziegler JL, Wabinga H, Katongole-Mbidde E, Boshoff C, Whitby D, Schulz T, Weiss RA, Jaffe HA and Moore PS. . 1996 Arch. Intern. Med. 156: 202–204.

  • Chen J, Ueda K, Sakakibara S, Okuno T and Yamanishi K. . 2000 J. Virol. 74: 8623–8634.

  • Dupin N, Grandadam M, Calvez V, Gorin I, Aubin JT, Harvard S, Lamy F, Leibowitch M, Huraux JM, Escande JP and Agut H. . 1995 Lancet 345: 761–762.

  • Frohman M. . 1990 PCR Protocols. Innis M, Gelfand DH, Sninsky JJ and White TJ. (eds). Academic Press: New York pp. 28–38.

  • Fujita T, Kimura Y, Miyamoto M, Barsoumian EL and Taniguchi T. . 1989 Nature 337: 270–272.

  • Gao SJ, Boshoff C, Jayachandra S, Weiss RA, Chang Y and Moore PS. . 1997 Oncogene 15: 1979–1985.

  • Gessain A, Sudaka A, Briere J, Fouchard N, Nicola MA, Rio B, Arborio M, Troussard X, Audouin J, Diebold J and de Thé G. . 1996 Blood 87: 414–416.

  • Harada H, Fujita T, Miyamoto M, Kimura Y, Maruyama M, Furia A, Miyata T and Taniguchi T. . 1989 Cell 58: 729–739.

  • Imam AM, Ackrill AM, Dale TC, Kerr IM and Stark GR. . 1990 Nucl. Acids Res. 18: 6573–6580.

  • Inagi R, Okuno T, Ito M, Chen J, Mori Y, Haque M, Zou P, Yagi H, Kiniwa S, Saida T, Ueyama Y, Hayashi K and Yamanishi K. . 1999 J. Hum. Virol. 2: 63–71.

  • Karcher DS and Alkan S. . 1995 N. Engl. J. Med. 333: 797–798.

  • Lebbé C, de Crémoux P, Rybojad M, Costa da Cunha C, Morel P and Calvo F. . 1995 Lancet 345: 1180.

  • Li M, Lee H, Guo J, Neipel F, Fleckenstein B, Ozato K and Jung JU. . 1998 J. Virol. 72: 5433–5440.

  • Moore PS, Boshoff C, Weiss RA and Chang Y. . 1996 Science 274: 1739–1744.

  • Moore PS and Chang Y. . 1995 N Engl. J. Med. 332: 1181–1185.

  • Nador RG, Cesarman E, Knowles DM and Said JW. . 1995 N. Engl. J. Med. 333: 943.

  • Pastore C, Gloghini A, Volpe G, Nomdedeu J, Leonardo E, Mazza U, Saglio G, Carbone A and Gaidano G. . 1995 Br. J. Haematol. 91: 918–920.

  • Pine R, Decker T, Kessler DS, Levy DE and Darnell JJ. . 1990 Mol. Cell. Biol. 10: 2448–2457.

  • Quandt K, Frech K, Karus H, Wingender E and Werner T. . 1995 Nucl. Acids Res. 23: 4878–4884.

  • Renne R, Zhong W, Herndier B, McGrath M, Abbey N, Kedes D and Ganem D. . 1996 Nat. Med. 2: 342–346.

  • Roan F, Zimring JC, Goodbown S and Offermann MK. . 1999 J. Gen. Virol. 80: 2205–2209.

  • Russo JJ, Bohenzky RA, Chien MC, Chen J, Yan M, Maddalena D, Parry JP, Peruzzi D, Edelman IS, Chang Y and Moore PS. . 1996 Proc. Natl. Acad. Sci. USA. 93: 14862–14867.

  • Sarid R, Flore O, Bohenzky RA, Chang Y and Moore PS. . 1998 J. Virol. 72: 1005–1012.

  • Schalling M, Ekman M, Kaaya EE, Linde A and Biberfeld P. . 1995 Nat. Med. 1: 707–708.

  • Soulier J, Grollet L, Oksenhendler E, Cacoub P, Cazals-Hatem D, Babinet P, d'Agay MF, Clauvel JP, Raphael M, Degos L, et al. 1995 Blood 86: 1276–1280.

  • Sun R, Lin SF, Staskus K, Gradoville L, Grogan E, Haase A and Miller G. . 1999 J. Virol. 73: 2232–2242.

  • Zimring JC, Goodbourn S and Offermann MK. . 1998 J. Virol. 72: 701–707.

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Acknowledgements

This work is supported by research grants to SJ Gao from the Howard Hughes Medical Institute through the University of Texas Health Science Center at San Antonio for the Research Resource Program for Medical Schools S/G 0#7, Elsa U Pardee Foundation, Association for International Cancer Research, and National Institute of Health (HL60604-01).

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Authors and Affiliations

  1. Department of Pediatrics, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, Texas, USA

    Xin-Ping Wang, Yan-Jin Zhang, Jian-Hong Deng, Hong-Yi Pan, Fu-Chun Zhou, Eduardo A Montalvo & Shou-Jiang Gao

  2. Department of Microbiology, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, Texas, USA

    Xin-Ping Wang, Yan-Jin Zhang, Jian-Hong Deng, Hong-Yi Pan, Fu-Chun Zhou, Eduardo A Montalvo & Shou-Jiang Gao

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Wang, XP., Zhang, YJ., Deng, JH. et al. Characterization of the promoter region of the viral interferon regulatory factor encoded by Kaposi's sarcoma-associated herpesvirus. Oncogene 20, 523–530 (2001). https://doi.org/10.1038/sj.onc.1204115

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