Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Paper
  • Published:

A transcription map of the minimally deleted region from 13q14 in B-cell chronic lymphocytic leukemia as defined by large scale sequencing of the 650 kb critical region

Oncogene volume 19, pages 5772–5780 (2000)Cite this article

Abstract

Extensive analysis of tumors has demonstrated homozygous and heterozygous deletions in chromosome region 13q14.3 in B-cell chronic lymphocytic leukemia (B-CLL), suggesting the site of a tumor suppressor gene. Since previous searches for this gene have not yielded any viable candidates, we now present the sequence of the BACs which span the minimally deleted approximately 650 kb region between markers D13S319 and D13S25. This sequence has allowed us to create the definitive transcription map for the region which reveals 93 ESTs and 12 Unigene clusters in this region. Using gene prediction programs, a further 19 potential genes are also identified. The genes show an asymmetrical distribution throughout the region with most of them clustering at the extreme ends. This sequencing effort provides for the definitive structure of the B-CLL deletion region and the identification of the vast majority of the potential candidate genes. Of all the genes identified, only three have homologies to known genes: two L1 repeat genes and rabbit epididymal protein 52. This 13q14.3 sequence provides the final substrate from which to characterize the B-CLL tumor suppressor gene.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3

Similar content being viewed by others

References

  • Altschul SF, Gish W, Miller W, Myers EW and Lipman DJ. . 1990 J. Mol. Biol. 215: 403–410.

  • Bezieau S, Devilder MC, Rondeau G, Cadoret E, Moisan JP and Moreau I. . 1998 Genomics 52: 369–373.

  • Bienz N, Cardy DLN, Leyland JM and Hulten MA. . 1993 Br. J. Haematol. 85: 819–822.

  • Bodenteich A, Chissoe S, Wang YF and Roe BA. . 1993 Shotgun cloning as the strategy of choice to generate templates for high-throughput dideoxynucleotide sequencing. In: Venter JC (ed). Automated DNA Sequencing and Analysis Techniques. London: Academic Press. pp 42–50.

  • Bouyge-Moreau I, Rondeau G, Avet-Loiseau H, Andre MT, Bezieau S, Cherel M, Saleun S, Cadoret E, Shaikh T, De Angelis MM, Arcot S, Batzer M, Moisan JP and Devilder MC. . 1997 Genomics 46: 183–190.

  • Brandon CI, Heusner GL, Caudle AB and Fayrer-Hosken RA. . 1999 Theriogenology 52: 863–873.

  • Brown AG, Ross FM, Dunne EM, Steel MC and Weir-Thompson EM. . 1993 Nature Genet. 3: 67–72.

  • Bullrich F, Veronese ML, Kitada S, Jurlander J, Caligiuri MA, Reed JC and Croce CM. . 1996 Blood 88: 3109–3115.

  • Burge C and Karlin S. . 1997 J. Mol. Biol. 268: 78–94.

  • Chapman RM, Corcoran MM, Gardiner A, Hawthorn LA, Cowell JK and Oscier DG. . 1994 Oncogene 9: 1289–1293.

  • Chissoe SL, Bodenteich A, Wang YF, Wang YP, Burian D, Clifton SW, Crabtree J, Freeman A, Iyer K and Jian L. . 1995 Genomics 27: 67–82.

  • Escudier SM, Pereira-Leahy K, Drach JW, Weier HU, Goodacre AM, Cork A, Andreeff M and Keating M. . 1993 Blood 10: 2702–2707.

  • Ewing B and Green P. . 1998 Genome Res. 8: 186–194.

  • Ewing B, Hillier L, Wendl MC and Green P. . 1998 Genome Res. 8: 175–185.

  • Fitchett M, Griffiths MJ, Oscier DG, Johnson S and Seabright M. . 1987 Cancer Genet. Cytogenet. 24: 143–150.

  • Genetics Computer Group. . (1994). [8]. Madison, WI, Genetics Computer Group. Ref Type: Computer Program

  • Gordon D, Abajian C and Green P. . 1998 Genome Res. 8: 195–202.

  • Hawthorn LA, Chapman R, Oscier D and Cowell JK. . 1993 Oncogene 8: 1415–1419.

  • Hawthorn LA and Cowell JK. . 1998 Ann. Human Genet. 62: 410–419.

  • Hawthorn LA, Roberts T, Verlind TE, Kooy RF and Cowell JK. . 1995 Genomics 30: 425–430.

  • Henikoff S and Henikoff JG. . 1994 Genomics 19: 97–107.

  • Holmes SE, Dombroski BA, Krebs CM, Boehm CD and Kazazian Jr HH. . 1994 Nat. Genet. 7: 143–148.

  • Iuliusson G, Friberg K and Gahrton G. . 1988 Cancer 62: 500–506.

  • Kalachikov S, Migliazza A, Cayanis E, Fracchiolla NS, Bonaldo MF, Lawton L, Jelenc P, Ye X, Qu X, Chien M, Hauptschein R, Gaidano G, Vitolo U, Saglio G, Resegotti L, Brodjansky V, Yankovsky N, Zhang P, Soares MB, Russo J, Edelman IS, Efstratiadis A, Dalla-Favera R and Fischer SG. . 1997 Genomics 42: 369–377.

  • Kazazian Jr HH, Wong C, Youssoufian H, Scott AF, Phillips DG and Antonarakis SE. . 1988 Nature 332: 164–166.

  • Killian GJ, Chapman DA and Rogowski LA. . 1993 Biol. Reprod. 49: 1202–1207.

  • Kooy RF, Verlind E, Houwen RHJ, Shapiro DN, Hawthorn LA, Cowell JK, Scheffer H and Buys CHCM. . 1994 Eur. J. Hum. Genet. 2: 59–65.

  • Miki Y, Nishisho I, Horii A, Miyoshi Y, Utsunomiya J, Kinzler KW, Vogelstein B and Nakamura Y. . 1992 Cancer Res. 52: 643–645.

  • Morse B, Rotherg PG, South VJ, Spandorfer JM and Astrin SM. . 1988 Nature 333: 87–90.

  • Narita N, Nishio H, Kitoh Y, Ishikawa Y, Ishikawa Y, Minami R, Nakamura H and Matsuo M. . 1993 J. Clin. Invest. 91: 1862–1867.

  • Pan HQ, Wang YP, Chissoe SL, Bodenteich A, Wang Z, Iyer K, Clifton SW, Crabtree JS and Roe BA. . 1994 Genet. Anal. Tech. Appl. 11: 181–186.

  • Petersen LC, Lindquist LL, Church S and Kay NE. . 1992 Genes Chroms. Cancer 4: 273–280.

  • Pittman S and Catovsky D. . 1983 Cancer Genet. Cytogenet. 9: 355–365.

  • Roberts T, Chernova O and Cowell JK. . 1998 Human Molec. Genet. 7: 1169–1178.

  • Roe B, Crabtree J and Khan A. . 1996 DNA Isolation and Sequencing: Essential Techniques Series. New York: J Wiley and Sons.

  • Sassaman DM, Dombroski BA, Moran JV, Kimberland ML, Naas TP, DeBerardinis RJ, Gabriel A, Swergold GD and Kazazian Jr HH. . 1997 Nat. Genet. 16: 37–43.

  • Schuler GD, Boguski MS, Stewart EA, Stein LD, Gyapay G, Rice K, White RE, Rodriguez-Tome P, Agarwal A, Bajorek E, Bentolila S, Birren BB, Butler A, Castle AB, Chiannikulchai N, Chu A, Clee C, Cowles S, Day PJ and Dibling T. . 1996 Science 274: 540–546.

  • Worley KC, Wiese BA and Smith RF. . 1995 Genome Res. 5: 173–184.

  • Xu Y, Einstein JR, Mural RJ, Shah M and Uberbacher EC. . 1994 Second International Conference on Intelligent Systems for Molecular Biology. Stanford University, San Francisco, CA.

Download references

Acknowledgements

Support for this work was obtained from National Institutes of Health grants CA74190 and NHGRI grants HG00313 and HG01604, and the National Science Foundation EPSCoR program to BA Roe.

Author information

Authors and Affiliations

  1. Center for Molecular Genetics, Lerner Research Institute/ND40, Cleveland Clinic Foundation, Cleveland, Ohio, USA

    Eiko Kitamura, Guanfang Su, Khalid Sossey-Alaoui, Lesleyann Hawthorn & John K Cowell

  2. Department of Chemistry, University of Oklahoma, Norman, Oklahoma, USA

    Eda Malaj, Jennifer Lewis, HuaQin Pan & Bruce Roe

Authors
  1. Eiko Kitamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Guanfang Su
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Khalid Sossey-Alaoui
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Eda Malaj
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jennifer Lewis
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. HuaQin Pan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Lesleyann Hawthorn
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Bruce Roe
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. John K Cowell
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kitamura, E., Su, G., Sossey-Alaoui, K. et al. A transcription map of the minimally deleted region from 13q14 in B-cell chronic lymphocytic leukemia as defined by large scale sequencing of the 650 kb critical region. Oncogene 19, 5772–5780 (2000). https://doi.org/10.1038/sj.onc.1203978

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/sj.onc.1203978

Keywords

This article is cited by

Search

Advanced search

Quick links