Abstract
The targeted knockout of the c-myc gene from rat fibroblasts leads to a stable defect in cell proliferation. We used complex cDNA libraries expressed from retroviral vectors and an efficient sorting procedure to rapidly select for cDNAs that can restore the growth rate of c-myc deficient cells. All of the biologically active cDNAs contained either c-myc or N-myc, suggesting that no other cellular genes can effectively bypass the requirement for c-myc in fibroblast proliferation. This approach provides a powerful screening method for cell cycle changes in genetically defined systems.
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Acknowledgements
HO 15.19 cells were a kind gift from John Sedivy, Brown University. We are grateful to Heather Van Buskirk for the critical reading of the manuscript. This work was supported by a grant from the National Cancer Institute to MD Cole.
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Nikiforov, M., Kotenko, I., Petrenko, O. et al. Complementation of Myc-dependent cell proliferation by cDNA expression library screening. Oncogene 19, 4828–4831 (2000). https://doi.org/10.1038/sj.onc.1203880
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DOI: https://doi.org/10.1038/sj.onc.1203880
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