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  • Original Paper
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B-Myc is preferentially expressed in hormonally-controlled tissues and inhibits cellular proliferation

Oncogene volume 19, pages 4886–4895 (2000)Cite this article

Abstract

The myc family of genes plays an important role in several cellular processes including proliferation, apoptosis, differentiation, and transformation. B-myc, a relatively new and largely unstudied member of the myc family, encodes a protein that is highly homologous to the N-terminal transcriptional regulatory domain of c-Myc. Here, we show that high level B-myc expression is restricted to specific mouse tissues, primarily hormonally-controlled tissues, with the highest level of expression in the epididymis. We also report the identification of the endogenous B-Myc protein from mouse tissues. Like other Myc family proteins, B-Myc is a short-lived nuclear protein which is phosphorylated on residues Ser-60 and Ser-68. Rapid proteolysis of B-Myc occurs via the ubiquitin-proteasome pathway. Finally, we found that overexpression of B-Myc significantly slows the growth of Rat 1a fibroblasts and COS cells suggesting B-Myc functions as an inhibitor of cellular proliferation.

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Acknowledgements

We thank Jingyu Shi and Diane Page for excellent technical assistance. This work was supported by US Public Health Service grants CA47399 (SR Hann) and CA78888 (SR Hann) from the National Cancer Institute and HD33903 (GA Cornwall) from the NICHD.

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Author notes

  1. Mark A Gregory and Qiurong Xiao: MA Gregory and Q Xiao contributed equally to this work

Authors and Affiliations

  1. Department of Cell Biology, MCN C-2310, Vanderbilt University School of Medicine, Nashville, 37232-2175, Tennessee, TN, USA

    Mark A Gregory, Qiurong Xiao, Bart Lutterbach & Stephen R Hann

  2. Department of Cell Biology and Biochemistry, Texas Tech University Health Science Center, Lubbock, 79430, Texas, TX, USA

    Gail A Cornwall

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  1. Mark A Gregory
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Gregory, M., Xiao, Q., Cornwall, G. et al. B-Myc is preferentially expressed in hormonally-controlled tissues and inhibits cellular proliferation. Oncogene 19, 4886–4895 (2000). https://doi.org/10.1038/sj.onc.1203851

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