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Detection of spatial localization of Hst-1/Fgf-4 gene expression in brain and testis from adult mice

Oncogene volume 19, pages 3805–3810 (2000)Cite this article

Abstract

HST-1, a member of the fibroblast growth factor (FGF) family (FGF-4), has been shown to be a signaling molecule whose expression is essential for embryonic development. However, HST-1/FGF-4 expression has not been detected or reported in adult tissues so far analysed. To investigate whether there is a possible role of HST-1/FGF-4 in adult stage, we have carried out a highly sensitive RT–PCR analysis of Hst-1/Fgf-4 gene expression in adult mice tissues. Results show Hst-1/Fgf-4 gene expression in the nervous system, intestines, and testis of normal adult mice. In situ hybridization technique was used to localize Hst-1/Fgf-4 gene expression in the cerebellum and testis from 10-week-old mice. Cell type-specific gene expression was detected: Purkinje cells in the cerebellum and Sertoli cells in testis. These findings suggest that the Hst-1/Fgf-4 gene also plays an important role in adult tissues, and may offer insights into the biological significance of HST-1/FGF-4 in cerebellar and testicular functions.

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References

  • Bean AJ, Elde R, Cao YH, Oellig C, Tamminga C, Goldstein M, Pettersson RF and Hokfelt T. . 1991 Proc. Natl. Acad. Sci. USA 88: 10237–10241.

  • Cancilla B and Risbridger GP. . 1998 Biol. Reprod. 58: 1138–1145.

  • Collignon J, Sockanathan S, Hacker A, Cohen-Tannoudji M, Norris D, Rastan S, Stevanovic M, Goodfellow PN and Lovell-Badge R. . 1996 Development 122: 509–520.

  • Dahmane N and Altaba Ari. . 1999 Development 126: 3089–3100.

  • Drucker BJ and Goldfarb M. . 1993 Mech. Dev. 40: 155–163.

  • el-Husseini A el-D, Paterson JA, Shiu RP. . 1994 Mol. Cell Endocrinol. 104: 191–200.

  • Fujino I, Yamada N, Miyakawa A, Hasegawa M, Furuichi T and Mikoshiba K. . 1995 Cell Tissue Res. 280: 201–210.

  • Gomez-Pinilla F and Cotman CW. . 1993 Brain 606: 79–86.

  • Griswold M, Morales C and Sylvester S. . 1988 Oxford Rev. Reprod. Biol. 10: 124–161.

  • Guillou F, Monet-Kuntz C, Fountain I and Flechon JE. . 1990 Cell Tissue Res. 260: 395–401.

  • Hannon K, Kudla AJ, McAvoy MJ, Clase KL and Olwin BB. . 1996 J. Cell Biol. 132: 1151–1159.

  • Heikinheimo O, Dong KW, Lanzendorf SE, Gordon K, Toner JP and Gibbons WE. . 1995 Mol. Reprod. Dev. 42: 397–406.

  • Heuer JG, von Bartheld CS, Kinoshita Y, Evers PC and Bothwell M. . 1990 Neuron 5: 283–296.

  • Ishii Y, Fukuda K, Saiga H, Matsushita S and Yasugi S. . 1997 Dev. Growth Differ. 39: 643–653.

  • Khodakhah K and Armstrong CM. . 1997 Proc. Natl. Acad. Sci. USA 94: 14009–14014.

  • Konishi H, Ochiya T, Sakamoto H, Tsukamoto M, Saito I, Muto T, Sugimura T and Terada M. . 1995 J. Clin. Invest. 95: 1125–1130.

  • Konishi H, Ochiya T, Yasuda Y, Sakamoto H, Muto T, Sugimura T and Terada M. . 1996 Oncogene 13: 9–19.

  • Le Magueresse-Battistoni B, Wolff J, Morera AM and Benahmed M. . 1994 Endocrinology 135: 2404–2411.

  • Matsuda S, Okumura N, Yoshimura H, Koyama Y and Sakanaka M. . 1992 Neuroscience 50: 99–106.

  • Matsuda S, Ii Y, Desaki J, Yoshimura H, Okumura N and Sakanaka M. . 1994 Neuroscience 59: 651–662.

  • McGaugh JL. . 2000 Science 287: 248–251.

  • Nakamura S, Todo T, Motoi Y, Haga S, Aizawa T, Ueki A and Ikeda K. . 1999 Glia 28: 53–65.

  • Nishimura T, Utsunomiya Y, Hoshikawa M, Ohuchi H and Itoh N. . 1999 Biochim. Biophys. Acta 18: 148–151.

  • Niswander L and Martin GR. . 1992 Development 114: 755–768.

  • Niswander L and Martin GR. . 1993 Nature 361: 68–71.

  • Niswander L, Jeffrey S, Martin GR and Tickle C. . 1994 Nature 371: 609–612.

  • Ochiya T, Sakamoto H, Tsukamoto M, Sugimura T and Terada M. . 1995 J. Cell Biol. 130: 997–1003.

  • Orth JM and Boehm R. . 1990 Anat. Rec. 226: 320–327.

  • Ozawa K, Uruno T, Miyakawa K, Seo M and Imamura T. . 1996 Mol. Brain Res. 41: 279–288.

  • Paranko J Kajjajoki M, Pelliniemi LJ, Lehto V-P and Virtanen I. . 1986 Dev. Biol. 117: 35–44.

  • Sakamoto H, Ochiya T, Sato Y, Tsukamoto M, Konishi H, Saito I, Sugimura T and Terada M. . 1994 Proc. Natl. Acad. Sci. USA 91: 12368–12372.

  • Shamin H, Mahmood R, Logan C, Doherty P, Lumsden A and Mason I. . 1999 Development 126: 945–959.

  • Suzuki HR, Sakamoto H, Yoshida T, Sugimura T, Terada M and Solursh M. . 1992 Dev. Biol. 150: 219–222.

  • Theillet C, Le Roy X, De Lapeyriere O, Grosgeorges J, Adnane J, Raynaud SD, Simony-Lafontaine J, Goldfarb M, Escot C, Birnbaum D and Gaudray P. . 1989 Oncogene 4: 915–922.

  • Todo T, Kondo T, Nakamura S, Kirino T, Kurokawa T and Ikeda K. . 1998 Brain Res. 783: 179–187.

  • Tsuda H, Hirohashi S, Shimosato Y, Hirota T, Tsugane S, Yamamoto H, Miyajima N, Toyoshima K, Yamamoto T, Yokota J, Yopshida T, Sakamoto H and Terada M. . 1989 Cancer Res. 49: 3104–3108.

  • Yang Y and Niswander L. . 1995 Cell 24: 939–947.

  • Yoshida T, Tsutsumi M, Sakamoto H, Miyagawa K, Teshima S, Sugimura T and Terada M. . 1988 Biochen. Biophys. Res. Commun. 155: 1324–1329.

  • Yoshida T, Miyagawa K, Sakamoto H, Sugimura T and Terada M. . 1991 Methods Enzymol. 198: 124–138.

  • Yoshida T, Ishimaru K, Sakamoto H, Yokota J, Hirohashi S, Igarashi K, Sudo K and Terada M. . 1994 Cancer Let. 83: 261–268.

  • Yuan H, Corbi N, Basilico C and Dailey L. . 1995 Gene Dev. 9: 2635–2645.

  • Vandaele S, Nordquist DT, Feddersen RM, Tretjakoff I, Peterson AC and Orr HT. . 1991 Gene Dev. 5: 1136–1148.

  • Van Dissel-Emiliani FM, De Boer-Brouwer M and De Rooij DG. . 1996 Endocrinology 137: 647–654.

  • Wilcox BJ and Unnerstall JR. . 1991 Neuron 6: 397–409.

  • Wilkinson DG. . 1992 In situ hybridization: A practical approach. IRL Press: London.

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Acknowledgements

We gratefully acknowledge Drs Katsuhiko Mikoshiba (Department of Molecular Neurobiology, Institute of Medical Science, University of Tokyo, Japan) and Teiichi Furuichi (Brain Science Institute, The Institute of Physical and Chemical Research, Saitama, Japan) for their kind assistance. We thank Ms Maki Abe for her technical work. This work was supported in part by a Grant-in-Aid for the Second-Term Comprehensive 10-Year Strategy for Cancer Control from the Ministry of Health and Welfare in Japan.

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Authors and Affiliations

  1. National Cancer Center Research Institute, 1-1, Tsukiji 5-chome, Chuo-ku, 104-0045, Tokyo, Japan

    Hanako Yamamoto, Takahiro Ochiya, Hiromi Sakamoto & Masaaki Terada

  2. First Department of Surgery, Nara Medical University, Nara, 634-8522, Japan

    Yasushi Takahama

  3. Division of Biochemistry and Cell Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashi, Kodaira, 187-8502, Tokyo, Japan

    Yasuo Ishii & Noriko Osumi

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  1. Hanako Yamamoto
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Yamamoto, H., Ochiya, T., Takahama, Y. et al. Detection of spatial localization of Hst-1/Fgf-4 gene expression in brain and testis from adult mice. Oncogene 19, 3805–3810 (2000). https://doi.org/10.1038/sj.onc.1203752

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