Abstract
We recently isolated the RhoH/TTF gene by its fusion to the LAZ3/BCL6 gene, in a non-Hodgkin's lymphoma (NHL) cell line, which bore a t(3;4)(q27;p11–13) translocation. This gene encodes a novel Rho GTP-binding protein and is specifically expressed in hematopoietic tissues. We made its precise mapping at band 4p13, and described its partial genomic structure. Using fluorescence in situ hybridization and molecular analyses, we report here on the rearrangement of the RhoH/TTF gene, at band 4p13, in four cases of NHL with t(3;4)(q27;p13) translocation and its fusion to the LAZ3/BCL6 gene at band 3q27, in three of these cases. RT–PCR analysis of two cases allowed the detection of variable fusion transcripts emerging from the rearranged alleles, and in one case, a deregulated expression of both RhoH/TTF and LAZ3/BCL6 genes, by promoter substitution, was observed. We also show here another rearrangement of the RhoH/TTF gene in a patient with multiple myeloma and t(4;14)(p13;q32) translocation, with breakage within the IGH gene. It is the first report which describes the recurrent chromosomal alteration of a GTP-binding protein encoding gene, in patients with hematopoietic malignancies.
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References
Albagli O, Deweindt C, Bernardin F, Dhordain P, Quief S, Lantoine D, Kerckaert JP and Leprince D . 1995 Cell Growth Differ 6: 1495–1503
Akasaka T, Miura I, Takahashi N, Akasaka H, Yonetani N, Ohno H, Fukuhara S and Okuma M . 1997 Cancer Res 57: 7–12
Bajalica-Lagercrantz PS, Piehl F, Lagercrantz J, Lindhal J, Weber G, Kerckaert JP, Porwit-MacDonald A and Nordenskjöld M . 1997 Leukemia 11: 594–598
Bastard C, Deweindt C, Kerckaert JP, Lenormand B, Rossi A, Pezzella F, Fruchard C, Monconduit M and Tilly H . 1994 Blood 83: 2423–2427
Bergsagel PL, Chesi M, Nardini E, Brents LA, Kirby SL and Kuehl WM . 1996 Proc Natl Acad Sci USA 93: 13931–13936
Callet-Bauchu E, Rimokh R, Tigaud I, Pagès J, Gazzo S, Bastion Y, Sebban C, Magaud JP, Coiffier B and Felman P . 1996 Genes Chromosom Cancer 17: 185–190
Cattoretti G, Chang CC, Cechova K, Zhang J, Ye BH, Falini B, Louie DC, Offit K, Chaganti RS and Dalla-Favera R . 1995 Blood 86: 45–53
Chen W, Iida S, Louie DC, Dalla-Favera R and Chaganti RSK . 1998 Blood 91: 603–607
Chesi M, Nardini E, Brents LA, Schrock E, Ried T, Kuehl WM and Bergsagel PL . 1997 Nature Genet 16: 260–264
Chirgwin J, Przybyla A, McDonald R and Rutter W . 1979 Biochem 18: 5294–5299
Dallery E, Galiègue-Zouitina S, Collyn-d'Hooghe M, Quief S, Denis C, Hildebrand MP, Lantoine D, Deweindt C, Tilly H, Bastard C and Kerckaert JP . 1995 Oncogene 10: 2171–2178
Dallery-Prudhomme E, Roumier C, Denis C, Preudhomme C, Kerckaert JP and Galiègue-Zouitina S . 1997 Genomics 43: 89–94
Daudignon A, Bisiau H, Laï JL, Wetterwald M, Galiègue-Zouitina S, Morel P and Duthilleul P . 1999 Cancer Genet Cytogenet 111: 157–160
Dent AL, Shaffer AL, Yu X, Allman D and Staudt LM . 1997 Science 276: 589–592
Dhordain P, Albagli O, Ansieau S, Koken MHM, Deweindt C, Quief S, Lantoine D, Leutz A, Kerckaert JP and Leprince D . 1995 Oncogene 11: 2689–2697
Dhordain P, Albagli O, Lin RJ, Ansieau S, Quief S, Leutz A, Kerckaert JP, Evans RM and Leprince D . 1997 Proc Natl Acad Sci USA 94: 10762–10767
Dhordain P, Lin RJ, Quief S, Lantoine D, Kerckaert JP, Evans RM and Albagli O . 1998 Nucleic Acids Res 26: 4645–4651
Galiègue-Zouitina S, Quief S, Hildebrand MP, Denis C, Detourmignies L, Laï JL and Kerckaert JP . 1999 Genes Chromosomes Cancer 26: 97–105
Galiègue-Zouitina S, Quief S, Hildebrand MP, Denis C, Lecocq G, Collyn-d'Hooghe M, Bastard C, Yuille M, Dyer MJS and Kerckaert JP . 1996 Leukemia 10: 579–587
Habets GGM, Scholtes EHM, Zuydgeest D, van der Kammen RA, Stam JC, Berns A and Collard JG . 1994 Cell 77: 537–549
Hall A . 1998 Science 279: 509–514
ISCN 1991 Guidelines for Cancer Cytogenetics, Supplement to an International System for Human Cytogenetic Nomenclature Mitelman F ed S Karger Basel
Kerckaert JP, Deweindt C, Tilly H, Quief S, Lecocq G and Bastard C . 1993 Nature Genet 5: 66–70
Manolova Y, Manolov G, Kieler J, Levan A and Klein G . 1979 Hereditas 90: 5–1
Michiels F, Habets GGM, Stam JC, van der Kammen RA and Collard JG . 1995 Nature 375: 338–340
Motokura T, Bloom T, Kim HG, Jüppner H, Ruderman JV, Kronenberg HM and Arnold A . 1991 Nature 350: 512–515
Nobes CD and Hall A . 1995 Cell 81: 53–62
Qiu R-G, Abo A, McCormick F and Symons M . 1997 Mol Cell Biol 17: 3449–3458
Qiu R-G, Chen J, Kirn D, McCormick F and Symons M . 1995a Nature 374: 457–459
Qiu R-G, Chen J, McCormick F and Symons M . 1995b Proc Natl Acad Sci USA 92: 11781–11785
Rabbitts TH . 1994 Nature 372: 143–149
Reibel L, Dorseuil O, Stancou R, Bertoglio J and Gacon G . 1991 Biochem Biophys Res Comm 175: 451–458
Storski T, Wlodarski P, Daheron L, Salomoni P, Nieborowska-Skorska M, Majewski M, Wasik M and Calabretta B . 1998 Proc Natl Acad Sci USA 95: 11858–11862
Tsujimoto Y, Finger LR, Yunis J, Nowell PC and Croce CM . 1984 Science 266: 1097–1099
Wong CW and Privalsky ML . 1998 J Biol Chem 273: 27695–27702
Ye BH, Cattoretti G, Shen Q, Zhang J, Hawe N, de Waard R, Leung C, Nouri-Shirazi M, Orazi A, Chaganti RSK, Rothman P, Stall A, Pandolfi P-P and Dalla-Favera R . 1997 Nat Genet 16: 161–170
Ye BH, Chaganty S, Chang CC, Niu H, Corradini P, Chaganti RSK and Dalla-Favera R . 1995 EMBO J 14: 6209–6217
Ye BH, Rao PH, Chaganti RSK and Dalla-Favera R . 1993 Cancer Res 53: 2732–2735
Zandecki M, Laï JL and Facon T . 1996 Br J Haematol 94: 217–227
Acknowledgements
Drs M Wetterwald, E Auxenfants, P Morel, from Dunkerque, Seclin, and Lens hospitals, are gratefully acknowledged for providing patients 3, 4, and 2 samples, respectively. We thank Dr Marie-Christine Copin for the lymph nodes material. Drs M Dyer, L Coignet, and T Rabbitts are gratefully acknowledged for giving us the IgH cosmids. The photographic work of Marie-Claire Duvieuxbourg is gratefully acknowledged. This work was supported by grants from ‘The Ligue Nationale Française contre le Cancer’, Comité du Pas de Calais, ‘The Association pour la Recherche contre le Cancer (ARC)’, Comité du Nord, the ‘Fondation contre la Leucémie’ and from the ‘Institut National de la Santé et de la Recherche Médicale’ (INSERM), France. One of us, E Dallery-Prudhomme, was recipient of a fellowship from the ‘Association pour la Recherche contre le Cancer (ARC)’.
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Preudhomme, C., Roumier, C., Hildebrand, M. et al. Nonrandom 4p13 rearrangements of the RhoH/TTF gene, encoding a GTP-binding protein, in non-Hodgkin's lymphoma and multiple myeloma. Oncogene 19, 2023–2032 (2000). https://doi.org/10.1038/sj.onc.1203521
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DOI: https://doi.org/10.1038/sj.onc.1203521
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