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Epstein–Barr virus encoded nuclear protein EBNA-3 binds XAP-2, a protein associated with Hepatitis B virus X antigen

Oncogene volume 19, pages 1801–1806 (2000)Cite this article

Abstract

EBNA-3 (also called EBNA-3A) is one of the EBV encoded nuclear antigens that are necessary for B-cell transformation. EBNA-3 is known to target RBPs, nuclear proteins that also interacts with EBNA-2, EBNA-4 and EBNA-6. In order to identify additional EBNA-3 targets, an EBV-transformed human lymphocyte cDNA library was screened in the yeast two-hybrid system with N-terminus truncated EBNA-3 that cannot interact with RBP-Jκ. A clone, encoding Xap-2 protein, a cellular partner of Hepatitis B virus X-antigen was isolated. This protein is also known as the p38 subunit of the aryl hydrocarbon receptor complex (ARA9). The specific binding to EBNA-3 was confirmed by showing that the GST-Xap-2 precipitated EBNA-3 from CV1 cells that were infected with recombinant vaccinia virus expressing EBNA-3. Deletion of the C-terminus of Xap-2 eliminated the binding. Fusion with green fluorescent protein showed that Xap-2 is preferentially cytoplasmic but translocates to the nucleus upon expression of EBNA-3.

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Acknowledgements

We thank Lena Norrenius for the excellent technical assistance and Klas G Wiman for the GST-ARF plasmids. This work was supported by Cancerfonden and by a matching grant from the Concern Foundation, Los Angeles, the Cancer Research Institute, New York.

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Authors and Affiliations

  1. Microbiology and Tumor Biology Centre (MTC) Karolinska Institute, S-171 77, Stockholm, Sweden

    Elena Kashuba, Vladimir Kashuba, Katja Pokrovskaja, George Klein & Laszlo Szekely

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  1. Elena Kashuba
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  2. Vladimir Kashuba
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  3. Katja Pokrovskaja
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  4. George Klein
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  5. Laszlo Szekely
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Kashuba, E., Kashuba, V., Pokrovskaja, K. et al. Epstein–Barr virus encoded nuclear protein EBNA-3 binds XAP-2, a protein associated with Hepatitis B virus X antigen . Oncogene 19, 1801–1806 (2000). https://doi.org/10.1038/sj.onc.1203501

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