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Krp1, a novel kelch related protein that is involved in pseudopod elongation in transformed cells

Oncogene volume 19, pages 1266–1276 (2000)Cite this article

Abstract

We have previously shown that the transcription factor AP-1 regulates the expression of genes which allow neoplastically transformed rat fibroblasts to become invasive. Searches for further AP-1 target genes led to the identification of a gene encoding a novel rat kelch family member, named kelch related protein 1 (Krp1). Kelch family members are characterized by a series of repeats at their carboxyl terminus and a BTB/POZ domain near their amino terminus. Rat Krp1 has a primarily cytoplasmic localization, and a small fraction appears to accumulate and co-localize with F-actin at membrane ruffle-like structures in the tips of pseudopodia. Overexpression of Krp1 in transformed rat fibroblasts led to the formation of dramatically elongated pseudopodia, while expression of truncated Krp1 polypeptides resulted in a reduction in the length of pseudopodia. We propose that the transformation-specific expression of Krp1 is required for pseudopod elongation, which are structures that are required for cell motility and invasion.

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Acknowledgements

We would like to thank Prof. JA Wyke and Drs MC Frame and G Stapleton for critical reading of the manuscript. We also thank P McHardy for assistance with microscopy. Thanks to E Schieble of the Beatson Institute for the tubulin monoclonal antibody. We gratefully acknowledge the Cancer Research Campaign for their financial support.

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Authors and Affiliations

  1. Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD

    Heather J Spence, Imogen Johnston, Karen Ewart, Sarah J Buchanan, Una Fitzgerald & Bradford W Ozanne

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  1. Heather J Spence
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  2. Imogen Johnston
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  3. Karen Ewart
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Spence, H., Johnston, I., Ewart, K. et al. Krp1, a novel kelch related protein that is involved in pseudopod elongation in transformed cells. Oncogene 19, 1266–1276 (2000). https://doi.org/10.1038/sj.onc.1203433

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