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  • Original Paper
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Identification of heterologous translocation partner genes fused to the BCL6 gene in diffuse large B-cell lymphomas: 5′-RACE and LA – PCR analyses of biopsy samples

Oncogene volume 18, pages 7994–7999 (1999)Cite this article

Abstract

In order to elucidate the molecular mechanism(s) for BCL6 translocation, we identified translocational partner genes by subjecting clinical biopsy samples from patients with non-Hodgkin's lymphoma to 5′-rapid amplification of cDNA ends (5′-RACE). Sequence analysis of the 5′-RACE product revealed that the BCL6 gene was fused to the J segment of the immunoglobulin heavy chain (IgH) gene in about half of the cases, but in the other half, it was fused to heterologous partners, including the MHC class II transactivator (CIITA), pim-1, eukaryotic initiation factor 4AII (eif4AII), transferrin receptor (TFRR) and ikaros genes. Since analyses using genomic long and accurate (LA) – PCR revealed that the breakpoints in the partner gene were confined to the first intron or the second exon in all cases, the promoter and the first exon of the BCL6 gene were replaced by the promoter and the first or both the first and second exon of the partner gene. The breakpoint flanking sequences had no recombination signal sequences (RSSs) or chi sequences and were homologous with the switch region only when the BCL6 gene was fused to the IgH gene, suggesting that BCL6 translocation cannot be explained solely by mistakes of V(D)J, or chi-mediated or class-switch recombination, but rather another mechanism may also be required to explain the molecular mechanism for the promiscuous BCL6 translocation.

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Acknowledgements

We thank Dr Tohru Miki for the gift of the ST-1 probe. We also thank Mis. Naoko Ishiguro for the assistance in preparing this manuscript.

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Author notes

  1. Shoko Yoshida and Yoshitaka Kaneita: S Yoshida and Y Kaneita contributed equally to this work

Authors and Affiliations

  1. Department of Pathology, Institute of Medical Science, The University of Tokyo, Tokyo, Japan

    Shoko Yoshida, Yoshitaka Kaneita, Yutaka Aoki, Shigeo Mori & Masatsugu Moriyama

  2. Laboratory of Chemotherapy, Aichi Cancer Institute, Nagoya, Japan

    Masao Seto

  3. Department of Molecular Biology, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Japan

    Masatsugu Moriyama

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  1. Shoko Yoshida
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Yoshida, S., Kaneita, Y., Aoki, Y. et al. Identification of heterologous translocation partner genes fused to the BCL6 gene in diffuse large B-cell lymphomas: 5′-RACE and LA – PCR analyses of biopsy samples. Oncogene 18, 7994–7999 (1999). https://doi.org/10.1038/sj.onc.1203293

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