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Indispensable role of the transcription factor PEBP2/CBF in angiogenic activity of a murine endothelial cell MSS31

Oncogene volume 19, pages 106–114 (2000)Cite this article

Abstract

Mice lacking the AML1/PEBP2αB/CBFa2 gene or PEBP2β/CBFb gene exhibit a defect in definitive hematopoiesis and die in utero because of hemorrhage in the central nervous system. Hematopoiesis in the embryo is considered to be tightly associated with vascular development. Here we examined whether PEBP2/CBF plays any role in angiogenesis besides that in definitive hematopoiesis. We found that AML1/PEBP2αB/CBFa2, PEBP2αA/CBFa1, and PEBP2β/CBFb were expressed in a murine endothelial cell line MSS31. The expression of these molecules as well as the DNA binding activity of PEBP2/CBF were augmented by angiogenic growth factors such as bFGF and VEGF. Moreover, the expression of PEBP2 α/CBFa protein in endothelial cells was confirmed at the site of angiogenesis in vivo. To further clarify the role of PEBP2/CBF in angiogenesis, we established permanent transfectants of PEBP2 β-MYH11 gene, one that interacts with the runt domain of the α subunit and deregulates PEBP2/CBF in a dominant interfering manner. Proliferation, migration, and tube formation of the PEBP2 β-MYH11 transfectants were significantly reduced in comparison with those activities of the mock transfectants. These results suggest that transcription factor PEBP2/CBF plays an important role in angiogenesis.

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Acknowledgements

This work was supported by a grant from the program Grants-in-Aid for Scientific Research on Priority Areas of the Japanese Ministry of Education, Science, Sports, and Culture.

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Authors and Affiliations

  1. Department of Vascular Biology, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan

    Katsunari Namba, Mayumi Abe, Sachiko Saito & Yasufumi Sato

  2. Department of Molecular Immunology, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan

    Masanobu Satake & Toshio Watanabe

  3. Department of Neurological Surgery, Okayama University Medical School, 2-5-1 Shikata-Cho, Okayama, 700-8558, Japan

    Katsunari Namba & Takashi Ohmoto

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  1. Katsunari Namba
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Namba, K., Abe, M., Saito, S. et al. Indispensable role of the transcription factor PEBP2/CBF in angiogenic activity of a murine endothelial cell MSS31. Oncogene 19, 106–114 (2000). https://doi.org/10.1038/sj.onc.1203257

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