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The physical association and phosphorylation of Cdc25C protein phosphatase by Prk

Oncogene volume 18, pages 6029–6036 (1999)Cite this article

Abstract

prk encodes a protein serine/threonine kinase involved in regulating M phase functions during the cell cycle. We have expressed His6-Prk and His6-Cdc25C proteins using the baculoviral vector expression system. Purified recombinant His6-Prk, but not a kinase-defective mutant His6-PrkK52R, is capable of strongly phosphorylating His6-Cdc25C in vitro. Co-immunoprecipitation and affinity column chromatography experiments demonstrate that GST-Prk and native Cdc25C interact. When co-infected with His6-Prk and His6-Cdc25C recombinant baculoviruses, sf-9 cells produce His6-Cdc25C antigen with an additional slower mobility band on denaturing polyacrylamide gels compared with cells infected with His6-Cdc25C baculovirus alone. In addition, His6-Cdc25C immunoprecipitated from sf-9 cells co-infected with His6-Prk and His6-Cdc25C baculoviruses, but not with His6-PrkK52R and His6-Cdc25C baculoviruses, contains a greatly enhanced kinase activity that phosphorylates His6-Cdc25C in vitro. Moreover, phosphopeptide mapping shows that His6-Prk phosphorylates His6-Cdc25C at two sites in vitro and that the major phosphorylation site co-migrates with the one that is phosphorylated in vivo in asynchonized cells. Further studies reveal that His6-Prk phosphorylates Cdc25C on serine216, a residue also phosphorylated by Chk1 and Chk2. Together, these observations strongly suggest that Prk's role in mitosis is at least partly mediated through direct regulation of Cdc25C.

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Acknowledgements

We are grateful to Yolanda Sanchez for providing us with GST-Cdc25C (200 – 256) and GST-Cdc25CS216A (200 – 256). We are also grateful to Helen Piwnica-Worms for providing us with His6-Cdc25C, GST-Wee1 recombinant baculoviruses and prokaryotic Cdc25C expression construct. We thank Akiko Kumagai and William Dunphy for providing us with a Xenopus Wee1 expression construct. Thanks also go to Ye Yiong and Konstantin Galaktionov for providing us with p34cdc2 expression construct and Cdc25C protein/antibody, respectively. This work was supported in part by United States Public Health Service Award RO1-74229.

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  1. Division of Hematology/Oncology, Department of Internal Medicine, University of Cincinnati College of Medicine;, ML-508, K-pavilion, 231 Bethesda Avenue, Cincinnati, 45267-0508, Ohio, OH, USA

    Bin Ouyang, Wenqing Li, Huiqi Pan, Juliana Meadows & Wei Dai

  2. German Cancer Research Center, Im Neuenheimer Feld 242, Heidelberg, Germany

    Ingrid Hoffmann

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Ouyang, B., Li, W., Pan, H. et al. The physical association and phosphorylation of Cdc25C protein phosphatase by Prk. Oncogene 18, 6029–6036 (1999). https://doi.org/10.1038/sj.onc.1202983

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