Abstract
E-cadherin is a cell-cell adhesion molecule expressed predominantly by epithelial cells. Reduction or loss of E-cadherin immunoreactivity has been associated with tumour progression in many epithelial cancers, including bladder carcinomas. The fibroblast growth factor receptor 2b (FGFR2b) recognized specifically by FGF7 is expressed only by epithelial cells. Recently, decreased expression of FGFR2b protein and mRNA was found to be associated with tumour progression in bladder carcinomas. The purpose of this investigation was to look for a possible relationship between E-cadherin and FGFR2b expression in bladder carcinomas. As decreased E-cadherin immunoreactivity was found to correlate directly with decreased expression at the mRNA level, the possible relationship between E-cadherin and FGFR2b was investigated at the mRNA level using semi-quantitative RT – PCR in 92 transitional cell carcinomas (TCCs) and four lymph node metastases. All tumours with low E-cadherin expression had low expression of FGFR2b, whereas tumours with low FGFR2b mRNA could express any level of E-cadherin mRNA. The same observation was equally valid for bladder and colon cancer cell lines suggesting that, besides bladder tumours, this relationship could apply to other carcinomas types. These results suggest that a relationship exists between the transcription of the E-cadherin and FGFR2b genes preventing high expression of FGFR2b where expression of E-cadherin is low. We suggest that reduced expression of FGFR2b in conjunction with decreased expression of E-cadherin may contribute to the aggressive behaviour attributable to high grade TCCs.
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Abbreviations
- FGFR:
-
fibroblast growth factor receptor
- TCC:
-
transitional cell carcinoma
- TBP:
-
TATA binding protein
- GAPDH:
-
glyceraldehyde-3-phosphate dehydrogenase
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Acknowledgements
This work has been supported in part by the Association Claude Bernard, Université Paris XII, Délégation à la Recherche Clinique AP-HP, CNRS, ARC 6455, Ligue Nationale Contre le Cancer (Comité de Paris), the Fondation de France and NIH grant 2RO1 CA 49417-06. S Gil-Diez de Medina is the recipient of a fellowship from the Ligue Contre le Cancer-Comité du Val de Marne. We thank Dr Bracke and Dr Mareel for the gift of the monoclonal antibody against E-cadherin and Dr Coulter for critical reading of the manuscript.
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De Medina, SD., Popov, Z., Chopin, D. et al. Relationship between E-cadherin and fibroblast growth factor receptor 2b expression in bladder carcinomas. Oncogene 18, 5722–5726 (1999). https://doi.org/10.1038/sj.onc.1202958
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DOI: https://doi.org/10.1038/sj.onc.1202958
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