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Chromosomal deletions occur in restricted regions of 5q in testicular germ cell cancer

Oncogene volume 18, pages 3277–3283 (1999)Cite this article

Abstract

Since the biologic behavior and molecular genetic changes observed in testicular germ cell cancer differ from those seen in more common epithelial tumors, it is likely that hitherto uncharacterized genes play a role in the development of germ cell tumors. Our previous work on testicular germ cell cancer suggested that chromosome 5q might contain one or more novel tumor suppressor genes that play a role in this malignancy. In this study, we performed a high resolution loss of heterozygosity (LOH) study of testicular cancer using 37 informative markers on chromosome 5. We detected allelic losses in 20/48 (42%) specimens and identified three common sites of loss on chromosome 5q14, 5q21 and 5q34-qter, defined respectively by minimal regions of deletion of ⩽1 cM, 10 cM and ∼20 (cM). Using an overlapping series of YACs and radiation hybrid mapping, we have constructed a physical map of the 5q14 deletion that should aid in the isolation and characterization of the putative tumor suppressor gene located therein.

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Acknowledgements

We would like to acknowledge the kind assistance of Dr Michael Jewett, of the Department of Surgery at The Toronto Hospital who supplied many of the testicular cancer specimens. We also thank Dr Steven Scherer of the Medical Research Council Genome Resource Facility at the Hospital for Sick Children, Toronto, for supplying the YAC clones. Finally, we gratefully acknowledge the patients who consented to provide material for this study. This work was supported by a Terry Fox Grant from the National Cancer Institute of Canada to D Hogg.

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Author notes

  1. Hong-Qi Peng

    Present address: General Oncology, Division of Medicine, Box 0092, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, Texas, 77030, USA

Authors and Affiliations

  1. Department of Medicine, Medical Sciences Building, University of Toronto, Toronto, M5S 1A8, Ontario, Canada

    Hong-Qi Peng, Ling Liu & David Hogg

  2. Department of Medical Biophysics, Medical Sciences Building, University of Toronto, Toronto, M5S 1A8, Ontario, Canada

    David Hogg

  3. Department of Medicine, The Toronto Hospital, Toronto, M5G 2C4, Ontario, Canada

    Paul E Goss & David Hogg

  4. Department of Pathology, The Toronto Hospital, Toronto, M5G 2C4, Ontario, Canada

    Denis Bailey

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  1. Hong-Qi Peng
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Peng, HQ., Liu, L., Goss, P. et al. Chromosomal deletions occur in restricted regions of 5q in testicular germ cell cancer. Oncogene 18, 3277–3283 (1999). https://doi.org/10.1038/sj.onc.1202662

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