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Identification of three distinct regions of allelic deletions on the short arm of chromosome 8 in hepatocellular carcinoma

Oncogene volume 18, pages 3127–3134 (1999)Cite this article

Abstract

The chromosome 8p is associated with a large number of allelic imbalances in epithelial tumors including hepatocellular carcinoma (HCC). However, no tumor suppressor gene has been identified so far in this particular region of the genome. To further clarify the pattern of allelic deletions on chromosome 8p in HCC, we have undertaken high-density polymorphic marker analysis of 109 paired normal and primary tumor samples using 40 microsatellites positioned every 2 cm in average throughout 8p. We found that 60% of the tumors exhibited loss of heterozygosity (LOH) at one or more loci at 8p with three distinct minimal deleted areas : a 13 cm region in the distal part of 8p21, a 9 cm area in the more proximal portion of 8p22 and a 5 cm area in 8p23. These data strongly suggest the presence of at least three novel tumor suppressor loci on 8p in hepatocellular carcinoma.

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Abbreviations

Al:

allelic imbalance

LOH:

loss of heterozygosity

MRL:

minimal region of loss

TSG:

tumor suppressor gene

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Acknowledgements

We thank C Bréchot, Z-Y Tang, L-X Qin, V. Mazzafero, R Raselli, B Terris, J Tran Van Nhieu and S Zafrani for providing us with tumor samples. We acknowledge F Lehembre for helpful discussions. This work was supported by grants from the Association pour la Recherche sur le Cancer and the Ligue contre le Cancer. P Pineau was supported by a grant from the Société de Secours des Amis de la Science. H Nagai was supported by a grant from the Fondation pour la Recherche Médicale.

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Author notes

  1. Hisaki Nagai

    Present address: Institute of Gerontology, Nippon Medical School, Kawasaki, Japan

  2. Gabor Gyapay & Jean Weissenbach

    Present address: Centre National de Séquençage (CNS), Evry, 91000, France

Authors and Affiliations

  1. Unité de Recombinaison & Expression Génétique, INSERM U163, Institut Pasteur, 28, rue du Docteur-Roux, 75724 Paris Cedex 15, France

    Pascal Pineau, Hisaki Nagai, Sylvie Prigent, Yu Wei, Pierre Tiollais, Marie-Annick Buendia & Anne Dejean

  2. Généthon, URA CNRS 1922, 1, rue de l'internationale, Evry, 91000, France

    Gabor Gyapay & Jean Weissenbach

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Pineau, P., Nagai, H., Prigent, S. et al. Identification of three distinct regions of allelic deletions on the short arm of chromosome 8 in hepatocellular carcinoma. Oncogene 18, 3127–3134 (1999). https://doi.org/10.1038/sj.onc.1202648

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