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11q23.1 and 11q25-qter YACs suppress tumour growth in vivo

Oncogene volume 18, pages 1157–1164 (1999)Cite this article

Abstract

Frequent allelic deletion at chromosome 11q22-q23.1 has been described in breast cancer and a number of other malignancies, suggesting putative tumour suppressor gene(s) within the ∼8 Mb deleted region. In addition, we recently described another locus, at the 11q25-qter region, frequently deleted in breast cancer, suggesting additional tumour suppressor gene(s) in this ∼2 Mb deleted region. An 11q YAC contig was accessed and three YACs, one containing the candidate gene ATM at 11q23.1, and two contiguous YACs (overlapping for ∼400 – 600 kb) overlying most of the 11q25 deleted region, were retrofitted with a G418 resistance marker and transfected into murine A9 fibrosarcoma cells. Selected A9 transfectant clones (and control untransfected and `irrelevant' alphoid YAC transfectant A9 clones) were assayed for in vivo tumorigenicity in athymic female Balb c-nu/nu mice. All the 11q YAC transfectant clones demonstrated significant tumour suppression compared to the control untransfected and `irrelevant' YAC transfected A9 cells. These results define two discrete tumour suppressor loci on chromosome 11q by functional complementation, one to a ∼1.2 Mb region on 11q23.1 (containing the ATM locus) and another to a ∼400 – 600 kb subterminal region on 11q25-qter.

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Authors and Affiliations

  1. Nuffield Department of Pathology and Bacteriology, University of Oxford, The John Radcliffe Hospital, Headington, OX3 9DU, Oxford, UK

    John Koreth, Christopher J Bakkenist, Nicholas CA Hunt & James O'D McGee

  2. Institute of Molecular Medicine, University of Oxford, The John Radcliffe Hospital, Headington, OX3 9DU, Oxford, UK

    Zoia Larin

  3. Wellcome Trust Centre for Human Genetics, University of Oxford, The John Radcliffe Hospital, Headington, OX3 9DU, Oxford, UK

    Michael R James

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  1. John Koreth
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Koreth, J., Bakkenist, C., Larin, Z. et al. 11q23.1 and 11q25-qter YACs suppress tumour growth in vivo. Oncogene 18, 1157–1164 (1999). https://doi.org/10.1038/sj.onc.1202372

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