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Resistance to avian leukosis virus lymphomagenesis occurs subsequent to proviral c-myc integration

Oncogene volume 18, pages 201–209 (1999)Cite this article

Abstract

Most chicken strains are highly susceptible to avian leukosis virus (ALV) induction of bursal lymphoma, involving proviral integration within the c-myc proto-oncogene, while certain strains are genetically resistant to lymphomagenesis. A nested PCR assay was developed to analyse the appearance of proviral c-myc integrations after ALV infection of lymphoma-susceptible birds, and to determine whether these integrations arise in lymphoma-resistant birds. Proviral c-myc integrations are detected in bursa and other tissues from 6 day-old lymphoma-susceptible birds infected as embryos. The abundance of bursal cells carrying these integrations increases roughly 40-fold by 35 days of age, indicating that these cells hyperproliferate within the bursal environment. Bursal cells with proviral c-myc integrations also arise soon after infection of lymphoma-resistant embryos. However, these cells expand much more slowly than cells from lymphoma-susceptible birds. Both strains show the same rate of viral infection, so that resistance to lymphomagenesis occurs at a step subsequent to proviral c-myc integration. Proviral c-erbB gene integrations arise at the same frequency in bursa and other tissues of both strains, and they do not increase in abundance during development. These findings indicate that the mechanism of resistance to lymphomagenesis involves specific inhibition of cells with proviral c-myc integrations within the bursa.

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Acknowledgements

We thank Laura Parks and the USDA Avian Disease and Oncology Laboratory (Lansing, MI) for providing fertile eggs and advice. This work was supported by NIH grant R01 CA68328 to A Ruddell.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Rochester, Rochester, New York, USA

    Kelly J Bird & Helen L Semus

  2. Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N. Mailstop C2-023, Seattle, PO Box 19024, Washington, USA

    Alanna Ruddell

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  1. Kelly J Bird
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  2. Helen L Semus
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  3. Alanna Ruddell
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Bird, K., Semus, H. & Ruddell, A. Resistance to avian leukosis virus lymphomagenesis occurs subsequent to proviral c-myc integration. Oncogene 18, 201–209 (1999). https://doi.org/10.1038/sj.onc.1202283

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