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Overexpression of p21waf1/cip1 arrests the growth of chicken embryo fibroblasts that overexpress E2F1

Abstract

P21waf1/cip1 is a potent inhibitor of cell cycle progression and can inhibit the growth of both normal cells and cells transformed by a number of oncogenes. However, the ability of p21waf1/cip1 to inhibit the growth of cells that overexpress the transcriptional transactivator E2F1 is controversial: it has been reported both that E2F1 can and cannot overcome the block in the cell cycle induced by p21waf1/cip1. To avoid the complications that arise when such experiments are done with permanent cell lines, we tested the effects of overexpressing p21waf1/cip1 and E2F1 in primary chicken embryo fibroblasts. In this system very high levels of E2F1 overexpression cause considerable apoptosis; however, the surviving cells still overexpress E2F1. These cells are transformed and their growth is blocked by overexpression of p21waf1/cip1.

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Givol, I., Givol, D. & Hughes, S. Overexpression of p21waf1/cip1 arrests the growth of chicken embryo fibroblasts that overexpress E2F1. Oncogene 16, 3115–3122 (1998). https://doi.org/10.1038/sj.onc.1201849

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