Abstract
We have cloned a cDNA for a novel human homolog of the Drosophila discs large (dlg) tumor suppressor protein, termed NE-dlg (neuronal and endocrine dlg). Northern blot analysis revealed that the gene is highly expressed in neuronal and endocrine tissues. Fluorescence in situ hybridization (FISH) and radiation hybrid mapping studies localized the NE-dlg gene to chromosome Xq13. We also found that the NE-dlg gene encoded a 100 kDa protein. Immunolocalization studies using an NE-dlg antibody showed that the protein tended to be expressed in non-proliferating cells, such as neurons, cells in Langerhans islets of the pancreas, myocytes of the heart muscles, and the prickle and functional layer cells of the esophageal epithelium. Proliferative cells, including various cultured cancer cell lines and basal cells in the esophageal epithelium, showed little expression of the NE-dlg protein. In addition, yeast two-hybrid screening and in vitro binding assays revealed that the NE-dlg interacted with the carboxyl-terminal region of the APC tumor suppressor protein. These data suggest that NE-dlg negatively regulates cell proliferation through its interaction with the APC protein.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Makino, K., Kuwahara, H., Masuko, N. et al. Cloning and characterization of NE-dlg: a novel human homolog of the Drosophila discs large (dlg) tumor suppressor protein interacts with the APC protein. Oncogene 14, 2425–2433 (1997). https://doi.org/10.1038/sj.onc.1201087
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/sj.onc.1201087
Keywords
This article is cited by
-
Gene expression profiling analysis reveals that DLG3 is down-regulated in glioblastoma
Journal of Neuro-Oncology (2014)
-
Polymorphisms in the DLG3 Gene is not Associated with Non-Syndromic Mental Retardation in the Chinese Han Population of Qin-Ba Mountain
Cellular and Molecular Neurobiology (2011)
-
The tumor suppressor WARTS activates the Omi / HtrA2-dependent pathway of cell death
Oncogene (2005)
-
The human protein Hugl-1 substitutes for Drosophila Lethal giant larvae tumour suppressor function in vivo
Oncogene (2004)
-
hScrib is a functional homologue of the Drosophila tumour suppressor Scribble
Oncogene (2003)