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Lack of association between the C825T polymorphism in G-protein beta-3 subunit and arterial blood pressure levels in cardiovascular disease free individuals: the ATTICA epidemiological study

Journal of Human Hypertension volume 20, pages 303–305 (2006)Cite this article

Hypertension is a complex multifactorial and polygenic disorder.1 Polymorphisms in many genes have been implicated for predisposition to hypertension.2, 3, 4 Siffert et al.4 first demonstrated a positive relationship between the polymorphism C825T in the GNB3 gene encoding the β3-subunit of heterotrimeric G proteins, with increased levels of blood pressure. However, there are other studies that have failed to show significant results regarding the relationship between the polymorphism C825T in the GNB3 gene and blood pressure levels.5, 6, 7 Therefore, we sought to evaluate the relationship between the C825T polymorphism in the GNB3 gene with the levels of blood pressure, and the prevalence of hypertension, in a Greek population-based sample of adults.

The ‘ATTICA’ study is a health and nutrition survey, which is being carried out in the province of Attica. During 2001–2002, 1514 men and 1528 women were, finally, enrolled. Of them, 375 (205 males and 170 females, aged 18–87 years old) were randomly selected and DNA extraction was performed. Genomic DNA was extracted from white blood cells. Polymerase chain reaction (PCR) was performed with the primers 5′-GCT GCC CAG GTC TGA TCC C-3′ (sense) and 5′-TGG GGA GGG TCC TTC CAG C-3′(antisense).4 PCR reactions began with denaturation at 93°C (30 s), annealing at 63°C (30 s), extension at 72°C and a final extension at 72°C (7 min). PCR products were restricted with 0.5 UbseDI (Fermentas), separated on 2.5% agarose gels and visualised under UV illumination. The T/T genotype yields one unrestricted band of 313 bp.

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Authors and Affiliations

  1. First Cardiology Department, School of Medicine, University of Athens, Athens, Greece

    C Pitsavos, L Papadimitriou, C Chrysohoou, J Skoumas & C Stefanadis

  2. Department of Nutrition – Dietetics, Harokopio University, Athens, Greece

    D Panagiotakos & G Dedoussis

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  1. C Pitsavos
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  2. L Papadimitriou
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  3. D Panagiotakos
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  4. C Chrysohoou
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  5. J Skoumas
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  6. G Dedoussis
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  7. C Stefanadis
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Correspondence to D Panagiotakos.

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Pitsavos, C., Papadimitriou, L., Panagiotakos, D. et al. Lack of association between the C825T polymorphism in G-protein beta-3 subunit and arterial blood pressure levels in cardiovascular disease free individuals: the ATTICA epidemiological study. J Hum Hypertens 20, 303–305 (2006). https://doi.org/10.1038/sj.jhh.1001985

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