FIGURE 5

The electrophoretically results of MCP-1 and VCAM-1 mRNA in untransfected VSMCs. -actin was used as an internal standard. Left, Representative electrophorogram. Right, Intensity of MCP-1 and VCAM-1 mRNA bands were counted with a bioimage analyzer and were normalized with intensity of GAPDH mRNA. (1) the normal control group, with DMEM/F12 serum-free medium; (2) the ox-LDL induced group, 50 mg/L ox-LDL with DMEM/F12 serum-free medium; (3) the ox-LDL and 50 mol/L DHEA group, 50 mg/L ox-LDL, 50 mol/L DHEA with DMEM/F12 serum-free medium; (4) the ox-LDL and 5 mol/L DHEA group, 50 mg/L ox-LDL, 5 mol/L DHEA with DMEM/F12 serum-free medium; (5) the ox-LDL and 0.5 mol/L DHEA group, 50 mg/L ox-LDL, 0.5 mol/L DHEA with DMEM/F12 serum-free medium; (6) the DHEA group, 5 mol/L with DMEM/F12 serum-free medium. The mRNA expression of MCP-1 and VCAM-1 in group 1 was the highest among groups. Group 2 and 4 were the lowest and had no significant difference. ^b P<0.05 vs group 2. ^e P<0.05 vs group 4.