Aim:

To investigate the effect of l-stepholidine (SPD) on the frequency of spontaneous excitatory postsynaptic currents (sEPSC) in the pyramidal cells between layers V and VI in the prelimbic cortex (PL).

Methods:

A whole-cell patch clamp in rat brain slices was used.

Results:

SPD significantly increased the frequency of sEPSC in a concentration-dependent manner. A selective D₁ dopamine receptor antagonist SCH23390 blocked SPD-mediated effects, whereas the D₁ agonist SKF38393, but not the D_2/3 antagonist sulpiride, mimicked SPD-mediated increase in the frequency of sEPSC. Moreover, both protein kinase A (PKA) inhibitor N-(2-[p-bromocinnamylamino]-ethyl)-5-isoquinolinesulfonamide hydrochloride and protein kinase C (PKC) inhibitor chelerythrine attenuated the effect of SPD on sEPSC.

Conclusion:

SPD elicits its effect on the frequency of sEPSC on the PL pyramidal cells via presynaptic D₁ receptors, and is dependent on PKA and PKC signaling pathways.

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