Original Article

Acta Pharmacologica Sinica (2006) 27, 229–236; doi:10.1111/j.1745-7254.2006.00243.x

Endocrine Pharmacology

Effects of astragaloside IV on pathogenesis of metabolic syndrome in vitro

Project supported by the Economic and Trade Commission of Zhejiang Province, the Key Laboratory of Chinese Medicine Screening, Exploitation and Medicinal Effectiveness Appraisal for Cardio-cerebral Vascular and Nervous System of Zhejiang Province, and the Key Laboratory for Biomedical Engineering of Ministry of China.

Ming-en Xu2,3, Shang-zhi Xiao3, Yong-hong Sun3, Yang Ou-yang3 and Xiao-xiang Zheng3

  1. 2Department of Biomedical Engineering, Hangzhou Dianzi University, Hangzhou 310018, China
  2. 3Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, China

Correspondence: Dr Ming-en Xu and Prof Xiao-xiang Zheng, E-mail: xumingen@hziee.edu.cn or xumingen@mail.tsinghua.edu.cn. Fax: 86-571-8795-1676. E-mail: zxx@mail.hz.zj.cn

Received 21 April 2005; Accepted 23 August 2005.

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Abstract

Aim:

 

To investigate the diverse pharmacological actions of astragaloside IV from the perspective of metabolic syndrome, and to investigate the effect of the drug on the pathogenesis of metabolic syndrome.

Methods:

 

Adipogenesis was used as an indicator of the effect of astragaloside IV on preadipocyte differentiation, and was measured by using an oil red O assay. Glucose uptake was determined by measuring the transport of [2-3H]-deoxyglucose into the cells. The concentrations of peroxisome proliferator-activated receptor-gamma(PPARgamma) and aP2 mRNA were determined by using reverse transcription-polymerase chain reaction. Apoptosis and viability loss of endothelial cells were detected by using flow cytometry and the WST-1 assay, respectively. Intracellular free Ca2+ was labeled with Fluo-3 AM and measured by using a laser scanning confocal microscope.

Results:

 

Astragaloside IV can significantly potentiate insulin-induced preadipocyte differentiation at concentrations of 3, 10, and 30 mug/mL, improve high glucose-induced insulin resistance in adipocytes at a concentration of 30 mug/mL, and prevent tumor necrosis factor (TNF)-alpha-induced apoptosis and viability loss at concentrations of 10 and 30 mug/mL, and 30 mug/mL, respectively, in endothelial cells. Furthermore, we found that these effects were partly due to the promotion of PPARgamma expression and to the inhibition of abnormal TNF-alpha-induced intracellular free Ca2+ accumulation in endothelial cells.

Conclusion:

 

The diverse pharmacological actions of astragaloside IV can all be linked to metabolic syndrome pathogenesis. Our study provides a new insight into the mechanism by which astragaloside IV exerts its effect.

Keywords:

astragaloside IV, metabolic syndrome X, insulin resistance, peroxisome proliferator-activated receptors, apoptosis, calcium

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