Original Article

Acta Pharmacologica Sinica (2006) 27, 1547–1552; doi:10.1111/j.1745-7254.2006.00461.x

Neuropharmacology

Intrathecal administration of Cav3.2 and Cav3.3 antisense oligonucleotide reverses tactile allodynia and thermal hyperalgesia in rats following chronic compression of dorsal root of ganglion

Xian-jie Wen1,2, Zhang-jun Li2, Zhi-xin Chen2, Zhi-yuan Fang3, Chen-xiang Yang1, Heng Li1 and Yin-ming Zeng2

  1. 1Department of Anesthesiology, the First People's Hospital of FoShan & Affiliated FoShan Hospital of Sun-Yat Sen University, Foshan 528000, China
  2. 2Jiangsu Province Institute of Anesthesiology, Xuzhou Medical College, Xuzhou 221002, China
  3. 3Department of Anesthesiology, the Municipal Hospital of Suzhou, Suzhou 215002, China

Correspondence: Prof Yin-ming Zeng, Fax: 86-516-570-8135. E-mail: zym-xzmc@163.com

Received 28 March 2006; Accepted 22 June 2006.

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Abstract

Aim:

 

The present study aimed to elucidate the role of T-subtype calcium channels (Cav3.1, Cav3.2, and Cav3.3) in the pathogenesis of neuropathic pain at spinal level.

Methods:

 

The chronic compression of the dorsal root ganglion (CCD) rat model was adopted. The antisense oligonucleotide of Cav3.1, Cav3.2, and Cav3.3 or normal saline (NS) were intrathecally administered twice per day from the first day to the fourth day after operation. Paw mechanical withdrawal threshold and paw thermal withdrawal latency were measured to evaluate the tactile allodynia and thermal hyperalgesia, respectively.

Results:

 

CCD rats developed reliable tactile allodynia and thermal hyperalgesia after operation. Intrathecal administration of antisense oligonucleotide of Cav3.2 and Cav3.3 significantly relieved tactile allodynia and thermal hyperalgesia in CCD rats, but not Cav3.1.

Conclusion:

 

Cav3.2 and Cav3.3 subtype calcium channels in the spinal cord may play an important role in the pathogenesis of neuropathic pain, which may contribute to the management of the neuropathic pain.

Keywords:

T-type calcium channels, neuropathic pain, spinal cord, antisense oligonucleotide

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