Nature Methods - Application Notes
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Functional isolation of staurosporine binding protein kinases using CCMS technology
Capture Compound mass spectrometry (CCMS) is an innovative technology to reduce biological sample complexity by selective isolation of targeted protein or enzyme families. Small synthetic molecules (Capture Compounds™) are used to interrogate native proteins. Using staurosporine as a selectivity function for a kinase-specific Capture Compound™ enables efficient complexity reduction of the proteome and allows discovery, isolation and profiling of functional kinases in biological samples. Kinase-specific Capture Compounds™ are available as ready-to-use caproKits™ for proteomic research.
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Application Notes Articles
Glen Mills
Generation of Human Umbilical Cord Stem Cells with a Bioreactor System
The Z® RP Technology developed by Zellwerk defines a new type of cell cultivation device. The unique rotating bed allows efficient cultivation of cell lines and primary cells. The Z® RP Technology enables users to expand all types of primary cells and yield larger amounts of these cells in vital status compared to common cultivation techniques. Human keratinocytes, chondrocytes, endothelial cells, hepatocytes e.g., as well as cells from many human tumor tissues can be expanded in the Z® RP. Detachment from the porous Sponceram® support is easily achieved.
Invitrogen
Rapid northern blotting (RNA transfer) with the iBlot®Dry Blotting System
Northern blotting1 is a technique for detection of specific RNA sequences that reveals information about RNA identity, size and abundance. The technique was developed by James Alwine and George Stark in 1977 at Stanford University. Total cellular RNA or mRNA is size-separated by denaturing agarose gel electrophoresis. The iBlot® Gel Transfer Device offers a novel method for electro-blotting macromolecules from separation gels to membranes in just 7 minutes.
Perkin Elmer
Image-based Quantification of Calcium Flux using the Opera
GPCRs are among the most heavily investigated drug targets in the pharmaceutical industry. The High Content Analysis application presented here is a useful and powerful tool for an image-based quantification of drug induced GPCR activation.
Epicentre Biotechnologies
Optimized library preparation method for next-generation sequencing
Go to Epicentre Biotechnologies > Full Text
The advent of next-generation sequencing has made possible genome analysis at previously unattainable depth. Roche, Illumina and Life Technologies, among others, have developedwell-established platforms for deep sequencing. Regardless of the instrument, one of the bottlenecks for next-generation sequencing is the amount of time and resources required for template and library preparation. Here we describe Epicentre's Nextera™ technology (covered by issued and/or pending patents), which counters this bottleneck and simplifies the sample preparation procedure.
Michelson Diagnostics
In vivo real-time optical coherence tomography imaging of Drosophila for cardiovascular research
Go to Michelson Diagnostics > Full Text
Optical coherence tomography (OCT) is a powerful imaging modality. OCT scanners, such as the Michelson Diagnostics EX1301 OCT microscope, uniquely provide real-time, in vivo, micrometer-scale images of biological tissue to a depth of 1 mm or more, allowing noninvasive study of dynamic tissue microstructure of living organisms at depths beyond the reach of standard microscopy. Here we describe the use of OCT to study the cardiovascular dynamics of Drosophila melanogaster.
febit GmbH
HybSelect: high-throughput access to genomic regions of interest for targeted next-generation sequencing
We present a technology for highly selective sequence capture using microarray hybridization for researchers who want to focus on relevant genomic regions in next-generation sequencing (NGS) experiments. The unique combination of the microfluidic Geniom Biochip® with the fully automated processing station Geniom RT Analyzer® thereby allows minimal hands-on time and high-throughput processing. Geniom Biochips® consist of eight channels containing individual capture probe arrays with complete sequence flexibility, ensuring applicability to any target sequence and scalability.
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