Nature Methods - Application Notes
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SENSOLUX® stand-alone version: noninvasive determination of pH and DO in shake flasks
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The determination of growth kinetic parameters during early process development is of crucial importance. The more information output is obtained, the more optimization strategies can be applied to generate highly productive cell lines. The new SENSOLUX® technology enables an optical and noninvasive measurement of pH and dissolved oxygen saturation in shake flasks. This technology provides a simple detection method suitable for effective final clone screening and medium optimization.
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BioTek
Automated Workflows for Luminex xMAP® Assays
Bead-based, multiplexing assays such as those provided by the Luminex xMAP® technology, have proven to be highly useful for biomarker identification and quantification from cells, tissues and body fluids. Applications include biological research, drug discovery and clinical diagnostics. The original technology based on the use of polystyrene microspheres tends to have arduous, manual work flows prone to cumulative systematic and random error that can lead to problematic precision for quantification. Here we demonstrate improvements in precision and ease-of-use through the use of BioTek microplate washers for both polystyrene and magnetic microspheres. Automation of workflows for partially-full microplates to full 96- and 384-well microplates can be performed.
Epicentre Biotechnologies
Next-generation sequencing library preparation: simultaneous fragmentation and tagging using in vitro transposition
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The advent of next-generation sequencing has made possible genome analysis at previously unattainable depth. Roche, Illumina and Life Technologies, among others, have developed well-established platforms for deep sequencing. Regardless of the instrument, one of the bottlenecks for next-generation sequencing is the amount of time and resources required for template and library preparation. Here we describe Epicentre's Nextera™ technology (covered by issued and/or pending patents), which counters this bottleneck and simplifies the sample preparation procedure.
Thermo Scientific
hMSC differentiation marker detection using Thermo Scientific Solaris™ qPCR Gene Expression Assays
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Human mesenchymal stem cells have become an important resource in developing strategies for regenerative therapies, owing to their ease of use and differentiation potential. Analytical tools, such as whole-genome expression array and validation with Solaris™ qPCR Assays, are essential to fully understand the key molecular events, such as microRNA-mediated gene modulation, that mark stem cell differentiation.
febit GmbH
HybSelect: high-throughput access to genomic regions of interest for targeted next-generation sequencing
We present a technology for highly selective sequence capture using microarray hybridization for researchers who want to focus on relevant genomic regions in next-generation sequencing (NGS) experiments. The unique combination of the microfluidic Geniom Biochip® with the fully automated processing station Geniom RT Analyzer® thereby allows minimal hands-on time and high-throughput processing. Geniom Biochips® consist of eight channels containing individual capture probe arrays with complete sequence flexibility, ensuring applicability to any target sequence and scalability.
Invitrogen
Rapid northern blotting (RNA transfer) with the iBlot®Dry Blotting System
Northern blotting1 is a technique for detection of specific RNA sequences that reveals information about RNA identity, size and abundance. The technique was developed by James Alwine and George Stark in 1977 at Stanford University. Total cellular RNA or mRNA is size-separated by denaturing agarose gel electrophoresis. The iBlot® Gel Transfer Device offers a novel method for electro-blotting macromolecules from separation gels to membranes in just 7 minutes.
Glen Mills
Generation of Human Umbilical Cord Stem Cells with a Bioreactor System
The Z® RP Technology developed by Zellwerk defines a new type of cell cultivation device. The unique rotating bed allows efficient cultivation of cell lines and primary cells. The Z® RP Technology enables users to expand all types of primary cells and yield larger amounts of these cells in vital status compared to common cultivation techniques. Human keratinocytes, chondrocytes, endothelial cells, hepatocytes e.g., as well as cells from many human tumor tissues can be expanded in the Z® RP.
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