FIGURE 3. Analysis of receptor internalization with ASSAYbuilder Physiology Analyst.
From the following Application Note:
High-content analysis with AxioVision ASSAYbuilder™
First published in Nature Methods 5, May 2008
(a) Raw image of stimulated cells (nuclei in blue, receptor in green). (b) Processed image after automatic object (cell) and spot (receptor) identification. (c) Scatter plot data derived from the image. Parameters such as the number or the total area of spots per cell can serve as a measure of receptor internalization. Nucleus shape, for example, identifies abnormal or apoptotic cells. The data point in red in the scatter plot (c) corresponds to the cell highlighted in yellow in the image (b). Scale bars, 10 
m.
