FIGURE 2. Cell-cycle analysis with ASSAYbuilder Cell Cycle Analyst.
From the following Application Note:
High-content analysis with AxioVision ASSAYbuilder™
First published in Nature Methods 5, May 2008
Images of untreated Huh-7 hepatocytes (left) and of the same cells treated for 24 h with a plant extract (right) are shown (nuclei in blue, mitochondria in green). The histograms show the results of a cell-cycle state analysis based on DNA content from multiple images taken from a 96-well plate. The control cell population shows a balanced pattern in the histogram where the majority of cells are in a diploid (2N) state (G1), in a tetraploid (4N) state (G2/M) and between these states. However, treated cells exhibited a dramatic trend toward a state above 4N. Scale bars, 50 
m.
