FIGURE 2. miRCURY LNA Arrays are highly specific for their miRNA targets.
From the following Application Note:
MicroRNA expression profiling on arrays enhanced with locked nucleic acids
First published in Nature Methods 3, April 2006
doi:10.1038/nmeth869
Two pools of synthetic miRNAs (2 fmol of each miRNA) were spiked into a complex background of yeast total RNA (1 
g/l) with hsa-mir-196a/hsa-mir-19a in one pool and hsa-mir-196b/hsa-mir-19b in the other pool. The a and b forms of has-mir-19 and has-mir-196 differ from each other by a single nucleotide. One pool was labeled with Hy3TM and the other pool was labeled with Hy5TM using the miRCURY LNA Array Labeling Kit. The reactions were pooled and hybridized onto miRCURY LNA Arrays. The perfect-match:mismatch ratios are in the range of 32–1,110.
