FIGURE 3. Mass spectrometry analysis showing Se-Met incorporation in cells grown with Overnight Express System 2.
From the following Application Note:
First published in Nature Methods 2, Pages 233-235, March 2005
doi:10.1038/nmeth0305-233
Cells expressing the target protein were grown in System 2 medium containing either Se-Met or Met. MS analysis demonstrates that the target protein produced with System 2 autoinduction incorporated Se-Met. a.i., ionization signal absolute intensity. (Spectra provided by the Mass Spectrometry/Bioanalytical Facility at the University of Wisconsin Biotechnology Center.)
