Special Feature
Method of the Year 2011
- Special Feature
Nature Methods' choice for Method of the Year 2011 is genome editing with engineered nucleases. This collection of articles—and the related video—highlights how the ability to use engineered nucleases to make precise, tailored and specific changes to coding and noncoding sequences of the genome, in cells and in organisms of many species, could revolutionize the study of gene function. The Methods to Watch bring together possible future choices for Method of the Year.
Editorial
Special feature: Method of the Year 2011
Method of the Year 2011
doi:10.1038/nmeth.1852
The ability to introduce targeted, tailored changes into the genomes of several species will make it feasible to ask more precise biological questions.
Abstract - | Full Text - Method of the Year 2010 | PDF (60 KB) - Method of the Year 2011
News Feature
Special feature: Method of the Year 2011
Gene-editing nucleases
Monya Baker
doi:10.1038/nmeth.1807
Precise ways to modify the genome arose from unexpected places. Monya Baker reports.
Abstract - | Full Text - Gene-editing nucleases | PDF (539 KB)
Primer
Special feature: Method of the Year 2011
Primer: genome editing with engineered nucleases
Natalie de Souza
doi:10.1038/nmeth.1848
A brief description of tools for targeted cleavage and tailored modification of genomes is presented.
Abstract - | Full Text - Primer: genome editing with engineered nucleases | PDF (432 KB)
Commentary
Special feature: Method of the Year 2011
Gene editing: not just for translation anymore -
Moira A McMahon, Meghdad Rahdar & Matthew Porteus
doi:10.1038/nmeth.1811
Engineered nucleases have advanced the field of gene therapy with the promise of targeted genome modification as a treatment for human diseases. Here we discuss why engineered nucleases are an exciting research tool for gene editing and consider their applications to a range of biological questions.
Abstract - | Full Text - Gene editing: not just for translation anymore | PDF (218 KB)
Special feature: Method of the Year 2011
Zinc-finger nucleases: how to play two good hands -
Mark Isalan
doi:10.1038/nmeth.1805
Zinc-finger nuclease dimers are more difficult to engineer than single DNA-binding domains, but the development of new methods could help.
Abstract - | Full Text - Zinc-finger nucleases: how to play two good hands | PDF (1282 KB)
Methods to Watch
Special feature: Method of the Year 2011
Single-cell methods -
Natalie de Souza
doi:10.1038/nmeth.1819
Improved single-cell methods are helping to unravel biological complexity.
Special feature: Method of the Year 2011
Functional genomic resources -
Nicole Rusk
doi:10.1038/nmeth.1820
Tools to manipulate murine genes on a genome-wide scale and to phenotype their effects in animals are maturing.
Abstract - | Full Text - Functional genomic resources | PDF (623 KB)
Special feature: Method of the Year 2011
Glycoproteomics-
Allison Doerr
doi:10.1038/nmeth.1821
Methods for tackling the enormously complex glycoproteome are sorely needed.
Special feature: Method of the Year 2011
Causal mutations in a haploid landscape -
Nicole Rusk
doi:10.1038/nmeth.1822
Sequencing a haploid genome and understanding the impact of its variants requires technical and computational improvements.
Abstract - | Full Text - Causal mutations in a haploid landscape | PDF (17064 KB)
Special feature: Method of the Year 2011
Imaging life with thin sheets of light-
Erika Pastrana
doi:10.1038/nmeth.1823
The revival of light-sheet microscopy opens new possibilities for the imaging of living processes.
Abstract - | Full Text - Imaging life with thin sheets of light | PDF (183 KB)
Special feature: Method of the Year 2011
Non–model organisms -
Tal Nawy
doi:10.1038/nmeth.1824
Next-generation sequencing is broadening the application of genetic and genomic studies to the panoply of life.
Special feature: Method of the Year 2011
Light-based electrophysiology -
Erika Pastrana
doi:10.1038/nmeth.1825
Genetically encoded voltage sensors are finally measuring up.
Abstract - | Full Text - Light-based electrophysiology | PDF (394 KB)
Special feature: Method of the Year 2011
RNA structures -
Petya V Krasteva
doi:10.1038/nmeth.1826
Accurate methods for RNA-structure determination are being developed.