To advance the toolset for controlling plant gene expression, we developed a CRISPR interference-based platform for the construction of synthetic Boolean logic gates that is functional in multiple plant species. These genetic circuits are programmable and reversible in nature, which will enable spatiotemporal control of plant responses to dynamic cues.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Gaber, R. et al. Designable DNA-binding domains enable construction of logic circuits in mammalian cells. Nat. Chem. Biol. 10, 203–208 (2014). This paper describes the construction of logic gates in human cell lines using TALE-based repressors.
Kiani, S. et al. CRISPR transcriptional repression devices and layered circuits in mammalian cells. Nat. Methods 11, 723–726 (2014). This study reports the construction of CRISPRi-based layered devices in human cell lines.
Nielsen, A. A. K. & Voigt, C. A. Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks. Mol. Syst. Biol. 10, 763 (2014). This paper describes the interfacing of CRISPRi-based circuits with host endogenous pathways in Escherichia coli.
Lloyd, J. P. B. et al. Synthetic memory circuits for stable cell reprogramming in plants. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01383-2 (2022). This research article describes the construction of memory genetic circuits in plants using recombinases.
Brophy, J. A. N. et al. Synthetic genetic circuits as a means of reprogramming plant roots. Science 377, 747–751 (2022). This paper reports the use of bacterial DNA-binding domains for construction of logic gates in plants.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Khan, M. A. et al. CRISPRi-based circuits to control gene expression in plants. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02236-w (2024).
Rights and permissions
About this article
Cite this article
Advancing programmable gene expression in plants using CRISPRi-based Boolean gates. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-024-02273-5
Published:
DOI: https://doi.org/10.1038/s41587-024-02273-5
