Featured
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Editorial
| Open AccessGenome editing
Recent advances in genome editing technologies have redefined our ability to probe and precisely edit the human genome and epigenome in vitro and in vivo. More specifically, RNA-guided CRISPR/Cas systems have revolutionized the field due to their simplicity in design and adaptability across biological systems. This Collection highlights results in CRISPR/Cas technology that increase the efficiency of precision genome editing, and allow genetic manipulation in model systems traditionally intractable to site-directed gene modification.
- Maura McGrail
- , Tetsushi Sakuma
- & Leonidas Bleris
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Article
| Open AccessAn efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters
- Jens Hamar
- & Dietmar Kültz
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Article
| Open AccessEfficient collection of a large number of mutations by mutagenesis of DNA damage response defective animals
- Yuji Suehiro
- , Sawako Yoshina
- & Shohei Mitani
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Article
| Open AccessCRISPY-BRED and CRISPY-BRIP: efficient bacteriophage engineering
- Katherine S. Wetzel
- , Carlos A. Guerrero-Bustamante
- & Graham F. Hatfull
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Article
| Open AccessGnrh2 maintains reproduction in fasting zebrafish through dynamic neuronal projection changes and regulation of gonadotropin synthesis, oogenesis, and reproductive behaviors
- Miranda Marvel
- , Berta Levavi-Sivan
- & Yonathan Zohar
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Article
| Open AccessFemale-to-male sex conversion in Ceratitis capitata by CRISPR/Cas9 HDR-induced point mutations in the sex determination gene transformer-2
- Roswitha A. Aumann
- , Irina Häcker
- & Marc F. Schetelig
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Article
| Open AccessRapid repair of human disease-specific single-nucleotide variants by One-SHOT genome editing
- Yuji Yokouchi
- , Shinichi Suzuki
- & Takumi Era
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Article
| Open AccessSystematized reporter assays reveal ZIC protein regulatory abilities are Subclass-specific and dependent upon transcription factor binding site context
- Jehangir N. Ahmed
- , Koula E. M. Diamand
- & Ruth M. Arkell
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Article
| Open AccessMultiFRAGing: Rapid and Simultaneous Genotyping of Multiple Alleles in a Single Reaction
- Cassidy Petree
- & Gaurav K. Varshney
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Article
| Open AccessCANCERSIGN: a user-friendly and robust tool for identification and classification of mutational signatures and patterns in cancer genomes
- Masroor Bayati
- , Hamid R. Rabiee
- & Hamid Alinejad-Rokny
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Article
| Open AccessIndel locations are determined by template polarity in highly efficient in vivo CRISPR/Cas9-mediated HDR in Atlantic salmon
- Anne Hege Straume
- , Erik Kjærner-Semb
- & Rolf Brudvik Edvardsen
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Article
| Open AccessPhenomics reveals a novel putative chloroplast fatty acid transporter in the marine diatom Skeletonema marinoi involved in temperature acclimation
- Oskar N. Johansson
- , Mats Töpel
- & Adrian K. Clarke
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Article
| Open AccessTargeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis)
- Kentaro Higuchi
- , Yukinori Kazeto
- & Koichiro Gen
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Article
| Open AccessA common variant of the pregnancy-associated plasma protein-A (PAPPA) gene encodes a protein with reduced proteolytic activity towards IGF-binding proteins
- Jane Alrø Bøtkjær
- , Pernille Rimmer Noer
- & Claus Yding Andersen
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Article
| Open AccessThe transporters SLC35A1 and SLC30A1 play opposite roles in cell survival upon VSV virus infection
- Anna Moskovskich
- , Ulrich Goldmann
- & Giulio Superti-Furga
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Article
| Open AccesslncRNA PSORS1C3 is regulated by glucocorticoids and fine-tunes OCT4 expression in non-pluripotent cells
- Fatemeh Mirzadeh Azad
- , Mahshid Malakootian
- & Seyed Javad Mowla
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Article
| Open AccessSkeletonema marinoi as a new genetic model for marine chain-forming diatoms
- Oskar N. Johansson
- , Mats Töpel
- & Adrian K. Clarke
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Article
| Open AccessIdentification of a Xist silencing domain by Tiling CRISPR
- Yang Wang
- , Yang Zhong
- & Jing Crystal Zhao
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Article
| Open AccessGenome reorganization of the GmSHMT gene family in soybean showed a lack of functional redundancy in resistance to soybean cyst nematode
- Naoufal Lakhssassi
- , Gunvant Patil
- & Khalid Meksem
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Article
| Open AccessEffects of CRISPR/Cas9 dosage on TICAM1 and RBL gene mutation rate, embryonic development, hatchability and fry survival in channel catfish
- Ahmed Elaswad
- , Karim Khalil
- & Rex Dunham
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Article
| Open AccessEditing the duplicated insulin-like growth factor binding protein-2b gene in rainbow trout (Oncorhynchus mykiss)
- Beth M. Cleveland
- , Ginnosuke Yamaguchi
- & Munetaka Shimizu
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Article
| Open AccessCattle with a precise, zygote-mediated deletion safely eliminate the major milk allergen beta-lactoglobulin
- Jingwei Wei
- , Stefan Wagner
- & Götz Laible
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Article
| Open AccessA Survey of Validation Strategies for CRISPR-Cas9 Editing
- Monica F. Sentmanat
- , Samuel T. Peters
- & Shondra M. Pruett-Miller
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Article
| Open AccessElectroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing
- Marie Teixeira
- , Bénédicte F. Py
- & Suzy Markossian
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Article
| Open AccessCombined shRNA over CRISPR/cas9 as a methodology to detect off-target effects and a potential compensatory mechanism
- Liat Peretz
- , Elazar Besser
- & Oded Behar
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Article
| Open AccessGenome-wide functional analysis reveals that autophagy is necessary for growth, sporulation, deoxynivalenol production and virulence in Fusarium graminearum
- Wuyun Lv
- , Chunyan Wang
- & Zhengyi Wang
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Article
| Open AccessFBN30 in wild Anopheles gambiae functions as a pathogen recognition molecule against clinically circulating Plasmodium falciparum in malaria endemic areas in Kenya
- Guodong Niu
- , Genwei Zhang
- & Jun Li
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Article
| Open AccessGeneration of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System
- Karim Khalil
- , Medhat Elayat
- & Rex Dunham
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Article
| Open AccessA haplotype in CFH family genes confers high risk of rare glomerular nephropathies
- Yin Ding
- , Weiwei Zhao
- & Huimei Chen
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Article
| Open AccessThe essential genomic landscape of the commensal Bifidobacterium breve UCC2003
- Lorena Ruiz
- , Francesca Bottacini
- & Douwe van Sinderen
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Article
| Open AccessDevelopment of an efficient vector system for gene knock-out and near in-cis gene complementation in the sugarcane smut fungus
- Shan Lu
- , Xiaorui Shen
- & Baoshan Chen
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Article
| Open AccessHomozygosity for the WRN Helicase-Inactivating Variant, R834C, does not confer a Werner syndrome clinical phenotype
- Ashwini S. Kamath-Loeb
- , Diego G. Zavala-van Rankin
- & Gabriela E. Mercado-Celis
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Article
| Open AccessOptimization of the production of knock-in alleles by CRISPR/Cas9 microinjection into the mouse zygote
- Aurélien Raveux
- , Sandrine Vandormael-Pournin
- & Michel Cohen-Tannoudji
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Article
| Open AccessIllegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9
- Shigeru Makino
- , Ryutaro Fukumura
- & Yoichi Gondo
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Article
| Open AccessCpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria
- Justin Ungerer
- & Himadri B. Pakrasi
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Article
| Open AccessIdentification of an evolutionary conserved structural loop that is required for the enzymatic and biological function of tryptophan 2,3-dioxygenase
- Helen Michels
- , Renée I. Seinstra
- & Ellen A. A. Nollen
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Article
| Open AccessFrom Function to Phenotype: Impaired DNA Binding and Clustering Correlates with Clinical Severity in Males with Missense Mutations in MECP2
- Taimoor I. Sheikh
- , Juan Ausió
- & John B. Vincent
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Article
| Open AccessSenescence: novel insight into DLX3 mutations leading to enhanced bone formation in Tricho-Dento-Osseous syndrome
- Na Zhao
- , Dong Han
- & Hailan Feng
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Article
| Open AccessFunctional and structural dissection of the tape measure protein of lactococcal phage TP901-1
- Jennifer Mahony
- , Mona Alqarni
- & Douwe van Sinderen
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| Open AccessA novel technique based on in vitro oocyte injection to improve CRISPR/Cas9 gene editing in zebrafish
- Shao-Lin Xie
- , Wan-Ping Bian
- & De-Sheng Pei
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Article
| Open AccessHeritable genome editing with CRISPR/Cas9 induces anosmia in a crop pest moth
- Fotini A. Koutroumpa
- , Christelle Monsempes
- & Emmanuelle Jacquin-Joly
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Article
| Open AccessPiggyBac transposon-based polyadenylation-signal trap for genome-wide mutagenesis in mice
- Limei Li
- , Peng Liu
- & Jian Fei
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Article
| Open AccessSesterterpene ophiobolin biosynthesis involving multiple gene clusters in Aspergillus ustus
- Hangzhen Chai
- , Ru Yin
- & Kui Hong
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Article
| Open AccessFunctional characterization of ABCB4 mutations found in progressive familial intrahepatic cholestasis type 3
- Hyo Jin Park
- , Tae Hee Kim
- & Ji Ha Choi
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Article
| Open AccessA CRISPR/Cas9 system adapted for gene editing in marine algae
- Marianne Nymark
- , Amit Kumar Sharma
- & Per Winge
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Article
| Open AccessTALENs-directed knockout of the full-length transcription factor Nrf1α that represses malignant behaviour of human hepatocellular carcinoma (HepG2) cells
- Yonggang Ren
- , Lu Qiu
- & Yiguo Zhang
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Article
| Open AccessBiallelic editing of a lamprey genome using the CRISPR/Cas9 system
- Yao Zu
- , Xushuai Zhang
- & Weiming Li
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Article
| Open AccessDistinct Biochemical Activities of Eyes absent During Drosophila Eye Development
- Meng Jin
- & Graeme Mardon
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Article
| Open AccessIdentification of OCTN2 variants and their association with phenotypes of Crohn’s disease in a Korean population
- Hyo Jin Park
- , Eun Suk Jung
- & Ji Ha Choi