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| Open AccessA new approach to obtain pure and active proteins from Lactococcus lactis protein aggregates
- L. Gifre-Renom
- , O. Cano-Garrido
- & E. Garcia-Fruitós
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| Open AccessTranscriptomic analyses of cacao cell suspensions in light and dark provide target genes for controlled flavonoid production
- Adriana M. Gallego
- , Luisa F. Rojas
- & Natalia Pabón-Mora
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| Open AccessAccelerated pharmaceutical protein development with integrated cell free expression, purification, and bioconjugation
- Dominique Richardson
- , Jaakko Itkonen
- & Marco G. Casteleijn
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| Open AccessCharacterization of the APETALA2/Ethylene-responsive factor (AP2/ERF) transcription factor family in sunflower
- Somayeh Najafi
- , Karim Sorkheh
- & Fatemeh Nasernakhaei
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| Open AccessCodon usage clusters correlation: towards protein solubility prediction in heterologous expression systems in E. coli
- Leonardo Pellizza
- , Clara Smal
- & Martín Arán
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| Open AccessEfficient production of human interferon beta in the white of eggs from ovalbumin gene–targeted hens
- Isao Oishi
- , Kyoko Yoshii
- & Takahiro Tagami
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| Open AccessSwitchable genome editing via genetic code expansion
- Toru Suzuki
- , Maki Asami
- & Anthony C. F. Perry
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| Open AccessRapid recombinant protein expression in cell-free extracts from human blood
- David Burgenson
- , Chandrasekhar Gurramkonda
- & Govind Rao
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| Open AccessDevelopment of bicistronic expression system for the enhanced and reliable production of recombinant proteins in Leuconostoc citreum
- Seung Hoon Jang
- , Ji Won Cha
- & Ki Jun Jeong
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| Open AccessDevelopment of a generic β-lactamase screening system for improved signal peptides for periplasmic targeting of recombinant proteins in Escherichia coli
- Tania Selas Castiñeiras
- , Steven G. Williams
- & Tim W. Overton
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| Open AccessHuman rhinovirus internal ribosome entry site element enhances transgene expression in transfected CHO-S cells
- Yu-rong Chai
- , Meng-meng Ge
- & Tian-yun Wang
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| Open AccessAlternative polyadenylation drives genome-to-phenome information detours in the AMPKα1 and AMPKα2 knockout mice
- Shuwen Zhang
- , Yangzi Zhang
- & Zhihua Jiang
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| Open AccessEvaluation of digital real-time PCR assay as a molecular diagnostic tool for single-cell analysis
- Chia-Hao Chang
- , Daxen Mau-Hsu
- & Tai-Horng Young
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Article
| Open AccessExpression, Purification and Characterization of the Human Cannabinoid 1 Receptor
- Srikrishnan Mallipeddi
- , Nikolai Zvonok
- & Alexandros Makriyannis
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Article
| Open AccessInsulin-like peptide 3 expressed in the silkworm possesses intrinsic disulfide bonds and full biological activity
- Takatsugu Miyazaki
- , Masaaki Ishizaki
- & Enoch Y. Park
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| Open AccessPurification and functional comparison of nine human Aquaporins produced in Saccharomyces cerevisiae for the purpose of biophysical characterization
- Frederik Bühring Bjørkskov
- , Simon Lyngaa Krabbe
- & Per Amstrup Pedersen
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| Open AccessAddition of a histone deacetylase inhibitor increases recombinant protein expression in Medicago truncatula cell cultures
- Rita B. Santos
- , Ana Sofia Pires
- & Rita Abranches
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Article
| Open AccessCell-free protein synthesis from non-growing, stressed Escherichia coli
- Jurek Failmezger
- , Michael Rauter
- & Martin Siemann-Herzberg
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Article
| Open AccessIdentification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects
- Xiao-Wei Yu
- , Wei-Hong Sun
- & Yan Xu
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Article
| Open AccessProkaryotic soluble expression and purification of bioactive human fibroblast growth factor 21 using maltose-binding protein
- Anh Ngoc Nguyen
- , Jung-A Song
- & Han Choe
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Article
| Open AccessProduction of recombinant human procollagen type I C-terminal propeptide and establishment of a sandwich ELISA for quantification
- Woo-Young Seo
- , Jeong-Ho Kim
- & Yong-Sung Kim
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| Open AccessA streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris
- Kate E. Royle
- & Karen Polizzi
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| Open AccessHeterologous expression and characterization of a new lipase from Pseudomonas fluorescens Pf0–1 and used for biodiesel production
- Wu Liu
- , Menggang Li
- & Yunjun Yan
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| Open AccessSerial-omics of P53−/−, Brca1−/− Mouse Breast Tumor and Normal Mammary Gland
- Susanne B. Breitkopf
- , Mateus De Oliveira Taveira
- & John M. Asara
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| Open AccessConventional and unconventional secretory proteins expressed with silkworm bombyxin signal peptide display functional fidelity
- Sungjo Park
- , D. Kent Arrell
- & Andre Terzic
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| Open AccessBacillus SEVA siblings: A Golden Gate-based toolbox to create personalized integrative vectors for Bacillus subtilis
- Jara Radeck
- , Daniel Meyer
- & Thorsten Mascher
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| Open AccessNovel Application of Magnetic Protein: Convenient One-Step Purification and Immobilization of Proteins
- Min Jiang
- , Lujia Zhang
- & Dongzhi Wei
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| Open AccessSequence analysis of feline immunoglobulin mRNAs and the development of a felinized monoclonal antibody specific to feline panleukopenia virus
- Zhengchun Lu
- , Rebecca L. Tallmadge
- & John S. L. Parker
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| Open AccessSensitive and long-term monitoring of intracellular microRNAs using a non-integrating cytoplasmic RNA vector
- Masayuki Sano
- , Manami Ohtaka
- & Mahito Nakanishi
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| Open AccessAntibacterial and immunomodulatory activities of insect defensins-DLP2 and DLP4 against multidrug-resistant Staphylococcus aureus
- Zhanzhan Li
- , Ruoyu Mao
- & Jianhua Wang
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| Open AccessImprovement of a fermentation process for the production of two PfAMA1-DiCo-based malaria vaccine candidates in Pichia pastoris
- Robin Kastilan
- , Alexander Boes
- & Rainer Fischer
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Article
| Open AccessCell-free synthesis of functional antibodies using a coupled in vitro transcription-translation system based on CHO cell lysates
- M. Stech
- , O. Nikolaeva
- & S. Kubick
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| Open AccessHigh-yield production of “difficult-to-express” proteins in a continuous exchange cell-free system based on CHO cell lysates
- Lena Thoring
- , Srujan K. Dondapati
- & Stefan Kubick
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| Open AccessIn planta expression of hyperthermophilic enzymes as a strategy for accelerated lignocellulosic digestion
- Bilal Ahmad Mir
- , Alexander A. Myburg
- & Don A. Cowan
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Article
| Open AccessFunctional Characterization of Two Elongases of Very Long-Chain Fatty Acid from Tenebrio molitor L. (Coleoptera: Tenebrionidae)
- Tianxiang Zheng
- , Hongshuang Li
- & Dayu Zhang
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| Open AccessLarge-scale production of recombinant human lactoferrin from high-expression, marker-free transgenic cloned cows
- Ming Wang
- , Zhaolin Sun
- & Yunping Dai
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| Open AccessRETRACTED ARTICLE: Impact of different promoters, promoter mutation, and an enhancer on recombinant protein expression in CHO cells
- Wen Wang
- , Yan-long Jia
- & Tian-yun Wang
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| Open AccessPredicting synonymous codon usage and optimizing the heterologous gene for expression in E. coli
- Jian Tian
- , Yaru Yan
- & Yunliu Fan
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| Open AccessA method for rapid high-throughput biophysical analysis of proteins
- Albert Perez-Riba
- & Laura S. Itzhaki
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| Open AccessHigh copy and stable expression of the xylanase XynHB in Saccharomyces cerevisiae by rDNA-mediated integration
- Cheng Fang
- , Qinhong Wang
- & Yanhe Ma
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| Open AccessA novel secretion and online-cleavage strategy for production of cecropin A in Escherichia coli
- Meng Wang
- , Minhua Huang
- & Jufang Wang
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| Open AccessSingle vector non-leaky gene expression system for Drosophila melanogaster
- Arslan Akmammedov
- , Marco Geigges
- & Renato Paro
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| Open AccessProteomic profiling of the weed feverfew, a neglected pollen allergen source
- Isabel Pablos
- , Stephanie Eichhorn
- & Gabriele Gadermaier
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| Open AccessImprovement in the production of the human recombinant enzyme N-acetylgalactosamine-6-sulfatase (rhGALNS) in Escherichia coli using synthetic biology approaches
- Luis H. Reyes
- , Carolina Cardona
- & Carlos J. Alméciga-Díaz
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| Open AccessMolecular switching system using glycosylphosphatidylinositol to select cells highly expressing recombinant proteins
- Emmanuel Matabaro
- , Zeng’an He
- & Morihisa Fujita
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| Open AccessQualifying a eukaryotic cell-free system for fluorescence based GPCR analyses
- Anne Zemella
- , Solveig Grossmann
- & Stefan Kubick
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| Open AccessImpact of Module-X2 and Carbohydrate Binding Module-3 on the catalytic activity of associated glycoside hydrolases towards plant biomass
- Nandita Pasari
- , Nidhi Adlakha
- & Syed Shams Yazdani
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| Open AccessControlled functional expression of the bacteriocins pediocin PA-1 and bactofencin A in Escherichia coli
- Beatriz Mesa-Pereira
- , Paula M. O’Connor
- & R. Paul Ross
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| Open AccessRF1 attenuation enables efficient non-natural amino acid incorporation for production of homogeneous antibody drug conjugates
- Gang Yin
- , Heather T. Stephenson
- & Trevor J. Hallam