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Super-resolution microscopy includes a variety of microscopy techniques that increase the resolving ability of a light microscope well beyond the classical limits dictated by the diffraction barrier.
The authors introduce ZS-DeconvNet, an unsupervised computational super-resolution method for multiple types of microscopes, that enhances image resolution by more than 1.5 times over the diffraction limit with 10 times lower fluorescence than regular superresolution imaging conditions.
This study combines lattice light sheet microscopy and single molecule imaging to study protein dynamics and chromatin structure in live cells. The authors describe how nucleosomes and proteins move and are organised in relation to chromatin density.
Here, the authors use spot array illumination to enable mechanical-scan-free super-resolution microscopy with adjustable resolution and good effective field of view, demonstrating a platform for studying molecular interactions at the nanoscale.
Dynamins are required at nascent endosomes to promote membrane fission. Here, the authors use super-resolution microscopy to show that dynamin-1 recruitment relies on pre-existing nanoclusters and trapping of molecules laterally diffusing on the plasma membrane.
The buildup and operation of a custom single-molecule localization microscope with state-of-the-art performance and advanced features bridges the gap between entry-level open-source projects and costly commercial systems.
Cancer cells adjust the composition of their glycocalyx to increase its thickness and create a physical barrier that shields them from immune recognition and engagement.
We introduce GelMap, a flexible workflow for reporting deformations and anisotropy in expansion microscopy. By intrinsically calibrating the expansion hydrogel using a fluorescent grid that scales with expansion and deforms with anisotropy, GelMap enables the reliable quantification of expansion factors and correction of deformations.
An expansion microscopy technique called ChromExM offers detailed views into the organization chromatin and associated gene expression machinery in embryos.